Molecular, structural, and phylogenetic analyses of Taxus chinensis JAZs
Molecular, structural, and phylogenetic analyses of Taxus chinensis JAZs
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红豆杉 JAZ 的分子、结构和系统发育分析
DOI：
10.1016/j.gene.2017.04.005
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发表时间：
2017
期刊：
影响因子：
3.5
通讯作者：
Yu Longjiang
中科院分区：
文献类型：
--
作者：
Zhang Meng;Chen Ying;Nie Lin;Jin Xiaofei;Fu Chunhua;Yu Longjiang
红豆杉属是古老的裸子植物，能产生一种独特的次级代谢产物，即抗癌药物紫杉醇。JAZ蛋白是JA信号通路的关键调节因子，控制紫杉醇的生物合成。然而，红豆杉JAZ蛋白。研究得很差。本研究利用我们以前的转录组数据从红豆杉中克隆了9个JAZ基因，命名为TcJAZ 1-TcJAZ 9。在这9个TcJAZ蛋白中，8个含有Jas和TIFY结构域，并且TcJAZ 6的Jas结构域是不完整的。大多数TcJAZ和PsJAZ与AtJAZ和OsJAZ无关。系统发育分析将拟南芥、水稻、云杉和T. chinensis分为8个亚群，裸子植物JAZ分为V-VIII亚群，被子植物JAZ分为I-V亚群。在裸子植物JAZ蛋白中发现了三个亚群VI-VIII的基序，表明裸子植物JAZ蛋白具有与被子植物不同的进化过程。9个TcJAZ基因的表达谱分析表明，TcJAZ 2/3/8基因是关键的调控基因，表明它们在中国红豆杉中具有重要的作用。结果表明，裸子植物JAZs在分子结构上不同于被子植物JAZs。在TcJAZs和PsJAZs中发现了三个新的保守基序。本研究为红豆杉JA调控系统的研究奠定了基础。阐明JA信号通路在陆生植物中的意义。
Taxus spp. are ancient gymnosperms that produce a unique secondary metabolite, namely, taxol, an anticancer drug. JAZ proteins are key regulators of the JA signaling pathway, which control taxol biosynthesis. However, the JAZ proteins ofTaxusspp. are poorly studied. In this work, nine JAZ genes from Taxus chinensis were identified using our previous transcriptome data and named asTcJAZ1–TcJAZ9. Of these nine TcJAZ proteins, eight contain Jas and TIFY domains, and the Jas domain of TcJAZ6 is incomplete. Most TcJAZs and PsJAZs are not related to AtJAZs and OsJAZs. Phylogenetic analysis divided all JAZ proteins from Arabidopsis thaliana,Oryza sativa, Picea sitchensis, andT. chinensisinto eight subgroups; gymnosperms JAZs were classified into subgroups V–VIII, and angiosperm JAZs were categorized into subgroups I–V. Three motifs of subgroups VI–VIII were identified in gymnosperm JAZs, indicating that gymnosperm JAZ proteins exhibit a different evolutionary process from those of angiosperms. The expression patterns of nine TcJAZs showed that TcJAZ2/3/8 was a key regulator, indicating their important roles inT. chinensis. Results revealed that gymnosperm JAZs differ from angiosperm JAZs in terms of molecular structure. Three novel conserved motifs were found in TcJAZs and PsJAZs. This study provides a basis for research on JA regulatory system inTaxusspp. and for elucidating the significance of JA signaling pathway to land plants.