The role of rpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses
The role of rpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses
复制标题
rpoS在溶藻弧菌毒力调节和对不同应激反应中的作用
DOI：
10.1111/jfd.12972
复制
发表时间：
2019-05-01
影响因子：
2.5
通讯作者：
Yan, Qingpi
中科院分区：
文献类型：
--
作者：
Huang, Lixing;Guo, Lina;Yan, Qingpi
溶藻弧菌是一种主要的水产病原体，给水产养殖业造成巨大损失。已证明rpoS在几种细菌的应激反应和毒力中发挥各种重要作用。在我们之前的研究中，经Cu 2+、Pb 2+、Hg 2+和低pH处理后，通过RNA-seq评估，rpoS的表达水平下调，同时观察到粘附能力受损，表明rpoS可能在粘附调节中起作用。本研究利用RNAi技术敲低溶藻弧菌rpoS基因。与野生型溶藻弧菌相比，RNAi处理后的溶藻弧菌在粘附、生长、溶血、生物膜形成、运动和毒力等方面的能力均显著降低。同时，温度、盐度、pH值和饥饿对rpoS表达有显著影响。目前的数据表明，rpoS是溶藻弧菌毒力的关键调节因子;此外，rpoS调节细菌粘附以响应温度、pH和营养成分的变化。这有助于探讨其致病机理，为病害防治提供参考。
Vibrio alginolyticus is a leading aquatic pathogen, causing huge losses to aquaculture. rpoS has been proven to play a variety of important roles in stress response and virulence in several bacteria. In our previous study, upon treatment with Cu2+, Pb2+, Hg2+ and low pH, the expression levels of rpoS were downregulated as assessed by RNA-seq, while impaired adhesion ability was observed, indicating that rpoS might play roles in the regulation of adhesion. In the present study, the RNAi technology was used to knockdown rpoS in V. alginolyticus. In comparison with wild-type V. alginolyticus, RNAi-treated bacteria showed significantly impaired abilities of adhesion, growth, haemolytic, biofilm production, movement and virulence. Meanwhile, alterations of temperature, salinity, pH and starvation starkly affected rpoS expression. The present data suggested that rpoS is a critical regulator of virulence in V. alginolyticus; in addition, rpoS regulates bacterial adhesion in response to temperature, pH and nutrient content changes. These are helpful to explore its pathogenic mechanism and provide reference for disease control.