Metabolic engineering of Arabidopsis to produce nutritionally important DHA in seed oil
Metabolic engineering of Arabidopsis to produce nutritionally important DHA in seed oil
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DOI：
10.1071/fp05084
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发表时间：
2005-01-01
影响因子：
3
通讯作者：
Green, AG
中科院分区：
文献类型：
--
作者：
Robert, SS;Singh, SP;Green, AG
二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）是具有重要营养价值的长链（b> = C-20） omega-3多不饱和脂肪酸（omega -3 LC-PUFA），目前主要从海洋中获得。在拟南芥中，一组编码脂肪酸链延伸酶和去饱和酶的基因在C-18 PUFA前体合成LC-PUFA所需的基因得到了种子特异性表达。这导致了DHA的合成，这是最具营养价值的omega - 3 LC-PUFA。第一次在种子油中，连同其前体EPA和omega - 6 LC-PUFA花生四烯酸（ARA）。该组装途径利用了作用于酰基辅酶a底物的Delta 5和Delta 6去饱和酶，与之前报道的酰基pc去饱和酶相比，其LC-PUFA的合成水平更高。这表明开发陆地植物作为DHA和其他LC-PUFA的替代来源，以满足对这些营养物质日益增长的需求的潜力。
Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are nutritionally important long-chain (>= C-20) omega-3 polyunsaturated fatty acids (omega 3 LC-PUFA) currently obtained mainly from marine sources. A set of genes encoding the fatty acid chain elongation and desaturation enzymes required for the synthesis of LC-PUFA from their C-18 PUFA precursors was expressed seed-specifically in Arabidopsis thaliana. This resulted in the synthesis of DHA, the most nutritionally important omega 3 LC-PUFA, for the. rst time in seed oils, along with its precursor EPA and the omega 6 LC-PUFA arachidonic acid (ARA). The assembled pathway utilised Delta 5 and Delta 6 desaturases that operate on acyl-CoA substrates and led to higher levels of synthesis of LC-PUFA than previously reported with acyl-PC desaturases. This demonstrates the potential for development of land plants as alternative sources of DHA and other LC-PUFA to meet the growing demand for these nutrients.