Investigation of the biological effects of artificial perfusion using rat extracorporeal circulation model.
Investigation of the biological effects of artificial perfusion using rat extracorporeal circulation model.
复制标题
利用大鼠体外循环模型研究人工灌注的生物学效应。
DOI：
10.1109/embc.2014.6944619
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Tatsumi E.
中科院分区：
文献类型：
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作者：
Fujii Y;Shirai M;Inamori S;Takewa Y;Tatsumi E.
体外循环是心脏外科手术不可缺少的一环。由于临床研究的困难使知识不足，因此希望具有用于小动物的微型ECC系统。本研究旨在建立一个微型的体外循环系统，并将其应用于大鼠，以研究其生化变化。ECC系统由膜式氧合器（聚丙烯，0.03 m2）、管路（聚氯乙烯）和滚子泵组成。该系统的预充体积仅为15 ml。将大鼠分为假手术组和ECC组。ECC泵流量开始并维持在70 ml/kg/min。我们测量了血清细胞因子水平的肿瘤坏死因子-a，白细胞介素（IL）-6，IL-10，和生化标志物（乳酸脱氢酶，天冬氨酸转氨酶和丙氨酸转氨酶）开始ECC前，60，120分钟后。此外，我们还测量了左肺组织的湿干重（W/D）比值。ECC期间血压和Hb维持在80 mmHg和10 g/dl左右，ECC组血清细胞因子水平和生化指标较SHAM组显著升高。ECC组的W/D比值较SHAM组显著增加。这些数据表明ECC促进器官损伤和全身炎症反应。这种大鼠ECC模型被认为是等同于已经建立的人类ECC和有用的研究在人工灌注过程中的病理生理变化的机制。
Extracorporeal circulation (ECC) is indispensable for cardiac surgery. Since difficulty in clinical research keeps the knowledge insufficient, it is desirable to have a miniature ECC system for small animals. We aimed to establish a miniature ECC system and apply the system to the rat for investigating biochemical changes. The ECC system consisted of a membranous oxygenator (polypropylene, 0.03 m2), tubing line (polyvinyl chloride) and roller pump. Priming volume of this system is only 15 ml. Rats were divided into the SHAM group and the ECC group. ECC pump flow was initiated and maintained at 70 ml/kg/min. We measured the serum cytokine levels of tumor necrosis factor-a, interleukin (IL)-6, and IL-10, and biochemical markers (lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase) before, 60, and 120 min after the initiation of ECC. In addition, we measured the wet-to-dry weight (W/D) ratio of the left lung tissues. During ECC, blood pressure and Hb were maintained around 80 mmHg and 10g/dl, the serum cytokine levels and biochemical markers were significantly elevated in the ECC group compared with the SHAM group. The W/D ratio increased significantly more in the ECC group compared with that in the SHAM group. These data suggest that ECC promotes organ damages and systemic inflammatory response. This rat ECC model is considered to be equivalent to the already established human ECC and useful for studying the mechanism of pathophysiological changes during artificial perfusion.