High-throughput cellular microarray platforms: applications in drug discovery, toxicology and stem cell research.
High-throughput cellular microarray platforms: applications in drug discovery, toxicology and stem cell research.
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DOI：
10.1016/j.tibtech.2009.02.009
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发表时间：
2009-06
影响因子：
17.3
通讯作者：
Cabral JM
中科院分区：
文献类型：
--
作者：
Fernandes TG;Diogo MM;Clark DS;Dordick JS;Cabral JM
细胞微阵列是高通量筛选大量测试样本的强大实验工具。小型化增加了分析吞吐量，同时减少了试剂消耗和所需细胞数量，使这些系统在药物发现、毒理学、干细胞研究和潜在治疗中的广泛分析中具有吸引力。在这里，我们提供了可用于生成细胞微阵列的新兴技术的概述，并重点介绍了该领域的最新重大进展。这一新兴的多学科方法为组织工程和细胞疗法中干细胞的设计和控制提供了新的机会，并有望加快生物技术和制药行业的药物发现。
Cellular microarrays are powerful experimental tools for high-throughput screening of large numbers of test samples. Miniaturization increases assay throughput while reducing reagent consumption and the number of cells required, making these systems attractive for a wide range of assays in drug discovery, toxicology, stem cell research and potentially therapy. Here, we provide an overview of the emerging technologies that can be used to generate cellular microarrays, and we highlight recent significant advances in the field. This emerging and multidisciplinary approach offers new opportunities for the design and control of stem cells in tissue engineering and cellular therapies and promises to expedite drug discovery in the biotechnology and pharmaceutical industries.