THE INORGANIC NO3--NH4+ RATIO INFLUENCES PLANT-REGENERATION AND AUXIN SENSITIVITY IN PRIMARY CALLUS DERIVED FROM IMMATURE EMBRYOS OF INDICA RICE (ORYZA-SATIVA L)
THE INORGANIC NO3--NH4+ RATIO INFLUENCES PLANT-REGENERATION AND AUXIN SENSITIVITY IN PRIMARY CALLUS DERIVED FROM IMMATURE EMBRYOS OF INDICA RICE (ORYZA-SATIVA L)
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DOI：
10.1016/s0176-1617(11)80063-5
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发表时间：
1990-06-01
影响因子：
4.3
通讯作者：
HODGES, TK
中科院分区：
文献类型：
--
作者：
GRIMES, HD;HODGES, TK
我们研究了无机NO_3 ~-：NH_4 ~+的比例是否会影响籼稻愈伤组织再生植株以及这些愈伤组织对外源生长素源2，4-D的反应。测试了对应于通常用于水稻细胞培养的NO3-：NH 4+比率的各种NO3-：NH 4+比率。在2 mg/L 2，4-D上诱导愈伤组织后，植物再生得到广泛范围的NO3-：NH 4+比率（从85：15 [21，25 mM NO3-：3.75 mM NH 4 +]到50：50 [12.5 mM NO3-：12.5 mM NH 4 +]）的支持。然而，降低NO3-：NH 4+的比例从80：20（原始的基础培养基组成）到75：25导致植物高度和根生长的三倍增加。如NO3-：NH_4 ~+浓度进一步降低，芽变短变粗，根变短，次生根减少。NO_3 ~-：NH_4 ~+比例也影响愈伤组织对不同浓度2，4-D的反应。在NO3-：NH_4 ~+比为80：20时，2，4-D的剂量-反应曲线较强，而NO_3 ~-：NH_4 ~+比为50：50时，2，4-D的剂量-反应曲线较平缓。
We have investigated whether the inorganic NO3-: NH4+ ratio can influence indica rice plant regeneration from primary callus and the response of these calli to an exogenous auxin source, 2,4-D. Various NO3-: NH4+ ratios were tested that correspond to the NO3-: NH4+ ratios commonly used for rice cell culture. Plant regeneration, after callus inductions on 2 mg/L 2,4-D, was supported by a wide range of NO3-: NH4+ ratios (from 85: 15 [21,25 mM NO3-: 3.75 mM NH4+] to 50:50 [12.5 mM NO3-: 12.5 mM NH4+]). However, lowering the NO3-: NH4+ ratio from 80:20 (the original basal medium composition) to 75:25 resulted in a three-fold increase in plant height and root growth. As the NO3-: NH4+ was lowered even further the shoots became shorter and thicker while the roots became more truncated and formed fewer secondary roots. The NO3-: NH4+ ratio also affected the response of these calli to different concentrations of 2,4-D. At a NO3-: NH4+ ratio of 80:20 there was a strong dose-response curve to 2,4-D while at a NO3-: NH4+ ratio of 50:50 this dose-response curve was much flatter.