Living skin equivalents constructed using human amnions as a matrix
Living skin equivalents constructed using human amnions as a matrix
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DOI：
10.1016/j.jdermsci.2009.09.009
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发表时间：
2009-12-01
影响因子：
4.6
通讯作者：
Hashimoto, Koji
中科院分区：
文献类型：
--
作者：
Yang, Lujun;Shirakata, Yuji;Hashimoto, Koji
背景：活体皮肤替代物(LSE)被用于治疗烧伤创面、皮肤缺损和慢性创面，今天，几种生物材料被用作LSE的真皮基质。目的：以人羊膜为基质，构建一种新型羊膜基质。方法：对人羊膜进行去上皮化，观察其对角质形成细胞的黏附和增殖、成纤维细胞的生长和增殖的影响。将角质形成细胞种植于成纤维细胞填充的、去上皮化的AM上皮侧，构建了一种新的LSE，并进行了组织学观察。将LSE移植到裸鼠全层创面上，并进行组织学检查。结果：去上皮化AM支持角质形成细胞的黏附、增殖和成纤维细胞的生长。新的LSE具有良好的力学性能和良好的形态，具有分化良好的表皮和发达的基底膜。结论：以羊膜为基质的LSE形态良好，成本低，具有良好的力学性能，有望作为皮肤替代物用于临床。(C)2009年日本皮肤病研究学会。爱思唯尔爱尔兰有限公司出版。版权所有。
Background: Living skin equivalents (LSEs) are being used to treat burn wounds, skin defects, and chronic wounds, and today, several biomaterials are applied as dermal matrices in LSEs. The amnionic membrane (AM) is known to have useful properties as a dermal matrix and can be used to construct a LSE.Objective: To develop a new LSE with human AM as the matrix.Methods: Human AM was de-epithelialized and investigated to determine whether it supported keratinocyte adherence and proliferation, and fibroblast in-growth and proliferation. A new LSE was constructed by seeding keratinocytes on the epithelial side of fibroblast-populated, de-epithelialized AM and was investigated histologically. The LSE was transplanted onto a full-thickness wound on a nude mouse and a histological examination was conducted.Results: De-epithelialized AM supported the adherence and proliferation of keratinocytes and the ingrowth of fibroblasts. The new LSE demonstrated good mechanical properties and revealed good morphology, with a well differentiated epidermis and well developed basement membrane. The LSE grafts survived well on nude mice, showing good morphology.Conclusion: A LSE with amnions as a matrix exhibited good morphology, low cost, and good mechanical properties and may be useful as a skin substitute for clinical use. (C) 2009 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.