A 26-KDA OUTER-MEMBRANE PROTEIN, OMPK, COMMON TO VIBRIO SPECIES IS THE RECEPTOR FOR A BROAD-HOST-RANGE VIBRIOPHAGE, KVP40
A 26-KDA OUTER-MEMBRANE PROTEIN, OMPK, COMMON TO VIBRIO SPECIES IS THE RECEPTOR FOR A BROAD-HOST-RANGE VIBRIOPHAGE, KVP40
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DOI：
10.1111/j.1574-6968.1995.tb07342.x
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发表时间：
1995-01-01
影响因子：
2.1
通讯作者：
TANAKA, S
中科院分区：
文献类型：
--
作者：
INOUE, T;MATSUZAKI, S;TANAKA, S
KVP 40是一种广泛宿主范围的弧菌噬菌体，在至少八种弧菌和一种发光杆菌属物种的菌株上形成噬斑。一个自发的KVP 40抗性突变体，R4000，来自副溶血性弧菌1010缺乏一个26 kDa的外膜蛋白OmpK。KVP 40被1010制备的外膜和OmpK灭活，但不被R4000制备的外膜灭活。这些结果强烈表明OmpK是KVP 40的受体。使用抗OmpK兔血清的免疫印迹分析显示，OmpK或其分子量为25-29 kDa的同源物广泛分布于弧菌属和发光杆菌属菌株中，包括那些天然耐KVP 40的菌株。
KVP40 is a broad-host-range vibriophage forming plaques on strains of at least eight Vibrio and one Photobacterium species. A spontaneous KVP40-resistant mutant, R4000, derived from Vibrio parahaemolyticus 1010 lacked a 26-kDa outer membrane protein designated OmpK. KVP40 was inactivated by outer membrane and OmpK prepared from 1010, but not by outer membrane from R4000. These results strongly suggest that OmpK is the receptor for KVP40. Immunoblotting analyses using an anti-OmpK rabbit serum revealed that OmpK or its homologs of molecular masses 25-29 kDa were distributed widely among Vibrio and Photobacterium strains including those naturally resistant to KVP40.