MOLECULAR-STRUCTURE OF THE BILIN BINDING-PROTEIN (BBP) FROM PIERIS-BRASSICAE AFTER REFINEMENT AT 2.0-A RESOLUTION
MOLECULAR-STRUCTURE OF THE BILIN BINDING-PROTEIN (BBP) FROM PIERIS-BRASSICAE AFTER REFINEMENT AT 2.0-A RESOLUTION
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DOI：
10.1016/0022-2836(87)90296-8
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发表时间：
1987-12-05
影响因子：
5.6
通讯作者：
KAYSER, H
中科院分区：
文献类型：
--
作者：
HUBER, R;SCHNEIDER, M;KAYSER, H
对菜粉蝶（Pierisbrassicae）的胆色素结合蛋白（BBP）进行了氨基酸序列、光谱、性质和三维结构分析。通过同晶置换确定的晶体结构已经在2.0埃细化。（1. = 0.1 nm）分辨率，R值为0.20。不对称单元包含四个独立的BBP亚基。坐标差为0.25埃，与坐标中的估计误差雅阁。多肽链折叠的特征在于八链桶。连接环在桶的上端张开并打开它，而下端是封闭的。整体形状类似于花萼。胆绿素IX γ。发色团位于筒体上端的中央裂缝中。比拉特林部分呈环状螺旋几何形状，具有Z、Z、Z构型和syn、syn、syn构象。的几何形状是在雅阁的光谱特性，并允许圆二色谱带的符号和意义的bilatriene螺旋之间的相关性。BBP的折叠与视黄醇结合蛋白（RBP）有关，尽管RBP和BBP的氨基酸序列仅显示10%的同源性，但在初步分析中已经认识到。在枪管上端的环有很大的差异，而枪管中心和下端的部分紧密相连。BBP和RBP的配体胆绿素和视黄醇的位置也类似。
The bilin binding protein (BBP) from the insect Pieris brassicae has been analysed for amino acid sequence, spectral, properties and three-dimensional structures. The crystal structure that had been determined by isomorphous replacement has been refined at 2.0 .ANG. (1 .ANG. = 0.1 nm) resolution to an R-value of 0.20. The asymmetric unit contains four independent subunits of BBP. The co-ordinate differences are 0.25 .ANG., in accord with the estimated error in co-ordinates. The polypeptide chain fold is characterized by an eight-stranded barrel. The connecting loops splay out at the upper end of the barrel and open it, whilst the lower end is closed. The overall shape resembles a calyx. The biliverdin IX.gamma. chromophore is located in a central cleft at the upper end of the barrel. The bilatrine moiety is in cyclic helical geometry with configuration Z,Z,Z and conformation syn,syn,syn. The geometry is in accord with the spectral properties and permits a correlation between sign of the circular dichroism bands and sense of the bilatriene helices. The fold of BBP is related to retinol binding protein (RBP), as had been recognized in the preliminary analysis, although the amino acid sequences of RBP and BBP show only 10% homology. There are large differences in the loops at the upper end of the barrel, whilst the segments of the centre and the lower end of the barrel superimpose closely. The ligands of BBP and RBP, biliverdin and retinol, respectively, are also similarly located.