Enhancing the Cellular Production of Extracellular Vesicles for Developing Therapeutic Applications.
Enhancing the Cellular Production of Extracellular Vesicles for Developing Therapeutic Applications.
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DOI：
10.1007/s11095-022-03420-w
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发表时间：
2023-04
影响因子：
3.7
通讯作者：
He, Mei
中科院分区：
文献类型：
--
作者：
Erwin, Nina;Serafim, Maria Fernanda;He, Mei
细胞外囊泡(EVS)具有体积小、生物相容性高、载药量高、细胞靶向性强等优点，在治疗开发中具有广阔的应用前景。电动汽车越来越多地被探索用于药物递送等应用。然而，由于电动汽车的细胞分泌速度有限，无法实现大规模的临床应用。因此，大量的战略和研究努力致力于提高电动汽车的细胞分泌率。本文综述了探索不同方法提高EVS细胞产量的各种研究，包括电刺激、药物、电磁波、声波、剪切力、细胞饥饿、酒精、pH、热和遗传操作的应用。这些方法在增加电动汽车产量方面取得了成功，但必须仔细考虑，因为其中许多策略可能会改变电动汽车的特性和功能，而且导致电动汽车细胞产量增加的确切机制通常尚不清楚。此外，这些方法在增加EV分泌方面的有效性可能因细胞系和条件的不同而不同。进一步提高EV的生物生成分泌以用于治疗开发仍然是该领域的重要需求。
Extracellular vesicles (EVs) have various advantageous properties, including a small size, high biocompatibility, efficient cargo loading, and precise cell targeting ability, making them promising tools for therapeutic development. EVs have been increasingly explored for applications like drug delivery. However, due to limited cellular secretion rates of EVs, wide-scale clinical applications are not achievable. Therefore, substantial strategies and research efforts have been devoted to increasing cellular secretion rates of EVs. This review describes various studies exploring different methods to increase the cellular production of EVs, including the application of electrical stimulus, pharmacologic agents, electromagnetic waves, sound waves, shear stress, cell starvation, alcohol, pH, heat, and genetic manipulation. These methods have shown success in increasing EV production, but careful consideration must be given as many of these strategies may alter EV properties and functionalities, and the exact mechanisms causing the increase in cellular production of EVs is generally unknown. Additionally, the methods’ effectiveness in increasing EV secretion may diverge with different cell lines and conditions. Further advancements to enhance EV biogenesis secretion for therapeutic development is still a significant need in the field.
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