Increased synthesis of two polypeptides in area CA1 of the hippocampus in response to repetitive electrical stimulation.
Increased synthesis of two polypeptides in area CA1 of the hippocampus in response to repetitive electrical stimulation.
复制标题
响应重复的电刺激，海马 CA1 区两种多肽的合成增加。
DOI：
10.1016/0006-8993(91)90802-3
复制
发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Thompson,RF
中科院分区：
文献类型：
--
作者：
Uenishi,N;Shors,TJ;Finch,CE;Nichols,NR;Thompson,RF
在体外海马切片中，研究了在有和没有长期增强诱导的重复刺激下新合成多肽模式的变化。利用[35S]蛋氨酸标记多肽和二维凝胶电泳，我们检测到CA1中两种多肽（48 kDa和89 kDa）的合成速率随着Schaffer侧枝的重复刺激而增加。48 kDa多肽（pI ~ 6.6）的合成在高频刺激（100 Hz）下相对于来自未刺激切片的相同蛋白质（n= 14）增加240%，在低频刺激（1 Hz）下增加220% （n= 5）。阻断n-甲基-d-天冬氨酸（NMDA）受体诱导了180%的蛋白质，破伤风刺激后没有进一步增加。89 kDa双偶波（pI ~ 6.8）在高频刺激下增加150%，在低频刺激下增加140%。阻断NMDA受体也增加了该蛋白（为未刺激对照组的180%），并且在高频刺激后未观察到进一步增加。基于物理化学和电生理特性，这些蛋白质不能被识别为任何先前与长期增强或重复电刺激相关的蛋白质。
Changes in the pattern of newly synthesized polypeptides were investigated in the in vitro hippocampal slice following exposure to repetitive stimulation with and without the induction of long-term potentiation. Using [35S]methionine labeling of polypeptides and two-dimensional gel electrophoresis, we detected an increase in the rate of synthesis of two polypeptides (48 kDa and 89 kDa) in CA1 in response to repetitive stimulation of the Schaffer collaterals. The synthesis of the 48 kDa polypeptide (pI∼ 6.6) increased 240% in response to high-frequency stimulation (100 Hz) relative to the same protein from unstimulated slices (n= 14), and increased 220% in response to low-frequency stimulation (1 Hz) (n= 5). Blockade of then-methyl-d-aspartate (NMDA) receptor induced the protein 180%, with no further increase following tetanic stimulation. An 89 kDa doublet (pI∼ 6.8) increased 150% following high-frequency and 140% following low-frequency stimulation. Blockade of the NMDA receptor increased this protein as well (180% of the unstimulated control) and no further increase was observed following high-frequency stimulation. Based on physicochemical and electrophysiological properties, these proteins are not identifiable as any of those previously associated with long-term potentiation or repetitive electrical stimulation.