Vitamin A inhibition of keratinization in rat urinary bladder cancer cell line Nara Bladder Tumor No. 2 in meniscus gradient culture.
Vitamin A inhibition of keratinization in rat urinary bladder cancer cell line Nara Bladder Tumor No. 2 in meniscus gradient culture.
复制标题
半月板梯度培养中维生素 A 对大鼠膀胱癌细胞系奈良膀胱肿瘤 2 号角化的抑制作用。
作者：
K. Toyoshima;J. Leighton
从Wistar大鼠的膀胱癌建立的奈良膀胱肿瘤2号细胞系，当用含15或30%胎牛血清的Eagle基本必需培养基培养时，在半月板梯度培养中形成角化细胞和多细胞珠。首先用常规水平位置的试管制备汇合单层培养物。然后将培养物改变为垂直位置时，在梯度的有氧端几天内观察到细胞分层、堆积和聚集。垂直放置1周后出现角化。鳞状分化进行角蛋白珍珠的形成，从未观察到如此明显的现象，并在水平培养如此丰富。补充维生素A，低至1 IU/ml，添加到培养基中，并没有抑制堆积或聚集，但防止角化。维生素A对角质化的抑制作用是可逆的。从培养基中除去维生素A后，聚集体中的细胞进行角化。
The Nara Bladder Tumor No. 2 cell line, established from a urinary bladder carcinoma in the Wistar rat, formed keratinizing cells and multicellular pearls in meniscus gradient culture when fed with a medium of 15 or 30% fetal calf serum in Eagle's minimal essential medium. Confluent monolayer cultures were first prepared with the tubes in conventional horizontal position. When cultures were then changed to a vertical position, stratification, piling up, and aggregation of cells were observed in a few days at the aerobic end of the gradient. Keratinization appeared 1 week after the tubes were placed in vertical position. Squamous differentiation proceeded to the formation of keratin pearls, a phenomenon never observed so distinctly and in such abundance in horizontal culture. Supplements of vitamin A, as low as 1 IU/ml, added to the medium did not inhibit piling up or aggregation but did prevent keratinization. Inhibition of keratinization by vitamin A was reversible. After vitamin A was removed from the medium, the cells in the aggregates progressed to keratinization.