Burkholderia pseudomallei animal and human isolates from Malaysia exhibit different phenotypic characteristics
Burkholderia pseudomallei animal and human isolates from Malaysia exhibit different phenotypic characteristics
复制标题
DOI：
10.1016/j.diagmicrobio.2007.01.002
复制
发表时间：
2007-07-01
影响因子：
2.9
通讯作者：
Nathan, Sheila
中科院分区：
文献类型：
--
作者：
Lee, Song-Hua;Chong, Chan-Eng;Nathan, Sheila
类鼻疽伯克霍尔德氏菌是一种革兰氏阴性的土壤腐殖菌，是类鼻疽病的病原体，是一种严重的致死性传染病。不同的临床和动物分离株表现出不同的表型性状，如生长速率，菌落形态，抗菌素耐药性和毒力。本研究旨在深入了解4种临床动物和6种动物B之间的内在差异。从马来西亚分离的类鼻疽。10株B.将这些分离株与无毒力的伯克霍尔德氏菌泰国亚种沿着进行测序以确认这些分离株的身份。核苷酸序列分析表明，10个B.假鼻疽分离株彼此相同。然而，16 S rRNA编码基因的核苷酸序列差异似乎为B。类鼻疽和B.泰国特有的。所有B的增长率。通过在37 ℃下72小时产生生长曲线来测定类鼻疽假单胞菌分离株。发现分离物的生长速率不同，倍增时间为1.5 - 2.3 h。此外，B.当在阿什当培养基、脑心浸液琼脂和Luria-Bertani琼脂上生长时，假鼻疽分离株在9天的观察中显示出菌落形态的相当大的变化。药敏试验结果表明，80%的菌株为Cb-R Kn（R）Gm（R）Chl（S）Te-S。B的毒性。在B中评价了类鼻疽临床和动物分离株。假鼻疽易感BALB/c小鼠。大多数临床分离株为高毒力。然而，毒力与分离株来源无关，因为2种动物分离株也具有高毒力。(c)2007年爱思唯尔公司All rights reserved.
Burkholderia pseudomallei is a Gram-negative saprophytic soil bacterium, which is the etiologic agent of melioidosis, a severe and fatal infectious disease occurring in human and animals. Distinct clinical and animal isolates have been shown to exhibit differences in phenotypic trait such as growth rate, colony morphology, antimicrobial resistance, and virulence. This study was carried out to gain insight into the intrinsic differences between 4 clinical and 6 animal B. pseudomallei isolates from Malaysia. The 16S rRNA-encoding genes from these 10 isolates of B. pseudomallei were sequenced to confirm the identity of these isolates along with the avirulent Burkholderia thailandensis. The nucleotide sequences indicated that the 16S rRNA-encoding genes among the 10 B. pseudomallei isolates were identical to each other. However, the nucleotide sequence differences in the 16S rRNA-encoding genes appeared to be B. pseudomallei and B. thailandensis specific. The growth rate of all B. pseudomallei isolates was determined by generating growth curves at 37 degrees C for 72 h. The isolates were found to differ in growth rates with doubling time varying from 1.5 to 2.3 h. In addition, the B. pseudomallei isolates exhibited considerable variation in colony morphology when grown on Ashdown media, brain-heart infusion agar, and Luria-Bertani agar over 9 days of observation. Antimicrobial susceptibility tests indicated that 80 % of the isolates examined were Amp(R) Cb-R Kn(R) Gm(R) Chl(S) Te-S. Virulence of the B. pseudomallei clinical and animal isolates was evaluated in B. pseudomallei-susceptible BALB/c mice. Most of the clinical isolates were highly virulent. However, virulence did not correlate with isolate origin since 2 of the animal isolates were also highly virulent. (c) 2007 Elsevier Inc. All rights reserved.