Genome-Wide Association Study Reveals Candidate Genes Involved in Fruit Trait Variation in Persian Walnut (Juglans regia L.).
Genome-Wide Association Study Reveals Candidate Genes Involved in Fruit Trait Variation in Persian Walnut (Juglans regia L.).
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DOI：
10.3389/fpls.2020.607213
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发表时间：
2020
影响因子：
5.6
通讯作者：
Dirlewanger E
中科院分区：
文献类型：
--
作者：
Bernard A;Crabier J;Donkpegan ASL;Marrano A;Lheureux F;Dirlewanger E
阐明核桃果实品质性状的遗传决定因素对培育满足生产者和消费者需求的新品种具有重要意义。我们使用多位点模型对INRAE核桃种质收集的170份核桃进行了全基因组关联研究（GWAS），之前使用AxiomTM J. regia 700K SNP阵列进行了基因分型。我们对25个果实性状进行表型分析，这些性状与形态计量学、形状、体积、重量、易开裂性和营养成分有关。我们发现了60多个标记-性状关联（mta），包括14号染色体上一个与坚果面径、坚果体积和籽粒体积相关的高度显著SNP，以及5个与核桃重量相关的额外SNP。我们提出了几个与坚果特性有关的候选基因，例如编码β -半乳糖苷酶的基因，该基因与几个大小相关的性状有关，并且已知与其他物种的果实发育有关。我们也证实了最近由加州大学戴维斯分校报道的5号和11号染色体与坚果缝合强度的关联。本研究结果增强了对核桃果实品质相关重要农艺性状遗传控制的认识，为今后辅助选择分子标记的开发奠定了基础。
Elucidating the genetic determinants of fruit quality traits in walnut is essential to breed new cultivars meeting the producers and consumers’ needs. We conducted a genome-wide association study (GWAS) using multi-locus models in a panel of 170 accessions of Juglans regia from the INRAE walnut germplasm collection, previously genotyped using the AxiomTM J. regia 700K SNP array. We phenotyped the panel for 25 fruit traits related to morphometrics, shape, volume, weight, ease of cracking, and nutritional composition. We found more than 60 marker-trait associations (MTAs), including a highly significant SNP associated with nut face diameter, nut volume and kernel volume on chromosome 14, and 5 additional associations were detected for walnut weight. We proposed several candidate genes involved in nut characteristics, such as a gene coding for a beta-galactosidase linked to several size-related traits and known to be involved in fruit development in other species. We also confirmed associations on chromosomes 5 and 11 with nut suture strength, recently reported by the University of California, Davis. Our results enhance knowledge of the genetic control of important agronomic traits related to fruit quality in walnut, and pave the way for the development of molecular markers for future assisted selection.
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影响因子：
4.5
作者：
Liu X;Huang M;Fan B;Buckler ES;Zhang Z
通讯作者：
Zhang Z
影响因子：
4.6
作者：
Arab, Mohammad Mehdi;Marrano, Annarita;Vahdati, Kourosh
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Vahdati, Kourosh
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作者：
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通讯作者：
Husson, Francois
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4.4
作者：
Bernard, Anthony;Marrano, Annarita;Dirlewanger, Elisabeth
通讯作者：
Dirlewanger, Elisabeth