Estrogen priming modulates autoreceptor-mediated potentiation of dopamine uptake.
Estrogen priming modulates autoreceptor-mediated potentiation of dopamine uptake.
复制标题
雌激素启动调节自身受体介导的多巴胺摄取增强。
DOI：
10.1016/s0014-2999(00)00432-5
复制
发表时间：
2000
影响因子：
5
通讯作者：
Smith,B
中科院分区：
文献类型：
--
作者：
Thompson,TL;Moore,CC;Smith,B
在从卵巢切除大鼠的脑桥核制备的突触体制备物中，研究了生理剂量的雌激素（苯甲酸雌二醇，雌激素：48和24小时前10 μg）调节自身受体介导的多巴胺转运特性变化的能力。雌激素致敏动物的突触体中，喹吡罗（1-100 μM）介导的[3 H]多巴胺摄取增强作用减弱。氟哌啶醇（10 μM）可抑制基础摄取，并有效阻止去卵巢和雌激素预处理样本中喹吡罗的摄取增强作用。还检查了选择性蛋白磷酸酶抑制剂调节自身受体介导的多巴胺摄取增强的能力。预处理蛋白磷酸酶2B（溴氰菊酯，氯氰菊酯）或蛋白磷酸酶1（互变霉素）抑制剂衰减的基础和喹吡罗增强多巴胺的摄取卵巢切除，但不是雌激素引发的组织。这些数据表明，自体受体介导的多巴胺转运的激活可以调节生理剂量的雌激素和牵连的作用，蛋白磷酸化自体受体介导的增强多巴胺的摄取。
The ability of a physiological dose of estrogen (estradiol benzoate, estrogen: 10 μg 48 and 24 h prior) to modulate autoreceptor-mediated changes in dopamine transport properties was investigated in a synaptosomal preparation prepared from the nucleus accumbens of ovariectomized rats. Quinpirole (1–100 μM)-mediated potentiation of [3H]dopamine uptake was attenuated in synaptosomes from estrogen-primed animals. Haloperidol (10 μM) inhibited basal uptake and effectively prevented quinpirole potentiation of uptake in both ovariectomized and estrogen-primed samples. The ability of selective protein phosphatase inhibitors to modulate autoreceptor-mediated potentiation of dopamine uptake was also examined. Pretreatment with protein phosphatase 2B (deltamethrin, cypermethrin) or protein phosphatase 1 (tautomycin) inhibitors attenuated basal and quinpirole-potentiated dopamine uptake in ovariectomized but not estrogen-primed tissue. These data suggest that autoreceptor-mediated activation of dopamine transport can be regulated by physiological doses of estrogen and implicate a role for protein phosphorylation in autoreceptor-mediated potentiation of dopamine uptake.