Pectin-like acidic polysaccharide from Panax ginseng with selective antiadhesive activity against pathogenic bacteria
Pectin-like acidic polysaccharide from Panax ginseng with selective antiadhesive activity against pathogenic bacteria
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DOI：
10.1016/j.carres.2006.03.032
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发表时间：
2006-07-03
影响因子：
3.1
通讯作者：
Kim, Kyung Hyun
中科院分区：
文献类型：
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作者：
Lee, Ji-Hye;Shim, Jin Sun;Kim, Kyung Hyun
先前的研究表明，从人参根中纯化的酸性多糖对幽门螺杆菌粘附胃上皮细胞和牙龈卟啉单胞菌凝集红细胞的能力有抑制作用。本研究进一步研究了人参酸性多糖PG-F2，以表征其对放线菌、痤疮丙酸杆菌和金黄色葡萄球菌的抗粘附作用。最低抑菌浓度（MIC）为0.25 ~ 0.5 mg/mL。然而，结果显示PG-F2对嗜酸乳杆菌、大肠杆菌和表皮葡萄球菌没有抑制作用。PG-F2是一种果胶型多糖，平均分子量为1.2 x 10(4) Da，主要由半乳糖醛酸和葡萄糖醛酸组成，鼠李糖、阿拉伯糖和半乳糖为次要成分。通过化学或碳水解酶处理的PG-F2完全水解导致其抗粘附活性丧失，但通过果胶酶（EC）处理的水解有限。3.2.1.15)得到低聚糖部分，其活性与前体PG-F2相当（对幽门螺杆菌和牙龈卟啉卟啉菌的MIC约为0.01 mg/mL）。结果表明，PG-F2可能对病原菌有选择性的抗粘附作用，而对有益菌和共生菌没有作用。(c) 2006 Elsevier Ltd.版权所有。
Previous studies have revealed the inhibitory effects of an acidic polysaccharide purified from the root of Panax ginseng against the adhesion of Helicobacter pylori to gastric epithelial cells and the ability of Porphyromonas gingivalis to agglutinate erythrocytes. In this study, this acidic polysaccharide from P. ginseng, PG-F2, was investigated further, in order to characterize its antiadhesive effects against Actinobacillus actinomycetemcomitans, Propionibacterium acnes, and Staphylococcus aureus. The minimum inhibitory concentrations (MIC) were found to be in a range of 0.25-0.5 mg/mL. However, results showed no inhibitory effects of PG-F2 against Lactobacillus acidophilus, Escherichia coli, or Staphylococcus epidermidis. PG-F2 is a pectin-type polysaccharide with a mean MW of 1.2 x 10(4) Da, and consists primarily of galacturonic and glucuronic acids along with rhamnose, arabinose, and galactose as minor components. The complete hydrolysis of PG-F2 via chemical or carbohydrolase enzyme treatment resulted in the abrogation of its antiadhesive activity, but limited hydrolysis via treatment with pectinase (EC. 3.2.1.15) yielded an oligosaccharide fraction, with activity comparable to the precursor PG-F2 (the MIC of ca. 0.01 mg/mL against H. pylori and P. gingivalis). Our results suggest that PG-F2 may exert a selective antiadhesive effect against pathogenic bacteria, while having no effects on beneficial and commensal bacteria. (c) 2006 Elsevier Ltd. All rights reserved.