Mechanisms of cytotoxicity of nickel ions based on gene expression profiles
Mechanisms of cytotoxicity of nickel ions based on gene expression profiles
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基于基因表达谱的镍离子细胞毒性机制
DOI：
10.1016/j.biomaterials.2008.09.011
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发表时间：
2009-01-01
期刊：
影响因子：
14
通讯作者：
Lu, Zuhong
中科院分区：
文献类型：
--
作者：
Lue, Xiaoying;Bao, Xiang;Lu, Zuhong
本研究利用基因芯片技术从基因表达谱水平研究了Ni（II）对小鼠成纤维细胞（L-929）的细胞毒性作用。将L-929细胞在含200 μ mNi（II）的培养液中培养24、48和72 h后，分别检测L-929基因表达谱，并采用MTT法检测Ni（II）的细胞毒性。20个上调基因和19个下调基因在3个培养期均有差异表达。基因本体分析表明，L-929细胞响应Ni（II）涵盖了广泛的功能基因组，包括细胞生物学过程，分子功能和细胞成分。Ni（II）通过诱导细胞凋亡抑制细胞增殖和分化，影响细胞发育，影响胆固醇代谢，对细胞具有广泛的作用。(c)2008爱思唯尔有限公司版权所有。
This study investigated cytotoxic effects of Ni(II) to mouse fibroblast cells (L-929) on the level of gene expression profiles with cDNA microarray. The gene expression profiles of L-929 were detected after the cells were Cultured in the medium with 200 pm Ni(II) for 24, 48 and 72 h, respectively, and the cytotoxicity of Ni(II) was evaluated with methylthiazoltetrazolium (MTT) assay. 20 up-regulated genes and 19 down-regulated genes were differentially expressed in all three-culture periods. Gene ontology analysis showed that the L-929 cells which responded to Ni(II) covered a broad range of functional gene groups including cellular biological process, molecular function, and cellular component. Ni(II) has extensive effects on cells by inhibiting cell proliferation and differentiation through inducing cell apoptosis, affecting cell development and influencing cholesterol metabolism. (c) 2008 Elsevier Ltd. All rights reserved.