Fumaric acid, anti-thrombin substance from Rhizopus javanicus.
Fumaric acid, anti-thrombin substance from Rhizopus javanicus.
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富马酸，来自爪哇根霉的抗凝血酶物质。
DOI：
10.3177/jnsv.43.241
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发表时间：
1997
影响因子：
1.6
通讯作者：
M. Ohsugi
中科院分区：
文献类型：
--
作者：
T. Okamura;N. Horie;Y. Miyazaki;M. Ohsugi
从爪哇根霉（Rhizopus javanicus）发酵液中分离到一种抗凝血酶物质（M2）。M2的积累在孵育14至15天后达到最大峰值，然后下降。M2的产量为500 mg，来自1 L培养液。M2抑制凝血酶活性，在含有50 μ L 12.5 NIH单位/mL凝血酶和200 μ L 0.33%牛纤维蛋白原的反应混合物中，其50%抑制浓度为63 μ M。M2对凝血酶具有特异性活性，但对纤溶酶、组织型纤溶酶原激活剂、尿激酶、血浆激肽释放酶和腺激肽释放酶的反应较低。通过元素分析、FAB/MS、~ 1H-NMR、~（13）C-NMR和IR等方法确定了M2的结构为富马酸。
An anti-thrombin substance (M2) was isolated from a culture broth of Rhizopus javanicus. Accumulation of M2 reached a maximum peak after 14 to 15 days of incubation and then decreased. The yield of M2 was 500 mg from 1 L of culture broth. M2 inhibited thrombin activity, and its 50% inhibition concentration in a reaction mixture containing 50 microL of 12.5 NIH unit/mL thrombin and 200 microL of 0.33% bovine fibrinogen was 63 microM. M2 had a specific activity for thrombin, but it was less responsive to plasmin, tissue-type plasminogen activator, urokinase, plasma kallikrein and glandular kallikrein. The structure of M2 was identified as fumaric acid by elementary analysis, FAB/MS, 1H-NMR, 13C-NMR and IR spectra.