Loss of heterozygosity in human ovarian cancer on chromosome 19q
Loss of heterozygosity in human ovarian cancer on chromosome 19q
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DOI：
10.1006/gyno.1997.4709
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发表时间：
1997-07-01
影响因子：
4.7
通讯作者：
Liang, B
中科院分区：
文献类型：
--
作者：
Bicher, A;Ault, K;Liang, B
癌基因和抑癌基因的功能异常与许多人类恶性肿瘤有关。ERCC 1和ERCC 2的mRNA表达异常可能是上皮性卵巢癌和脑肿瘤的特征性表现。因此，我们对含有这些DNA修复基因的染色体19q13.2-q13.4进行了研究。D19 S246位于HRC和KLK 1之间的2 Mb片段上，杂合性缺失率为53%（8/15）。缺失区域的遗传物质在着丝粒和端粒都是保守的，该区域与三个DNA修复基因位于端粒，其中LIG 1为1 Mb，ERCC 1和ERCC 2为3.5Mb和4.0Mb。这些结果代表了人类卵巢癌染色体19 q13.2-q13.4上洛合性缺失率的生物学意义的首次报道。(C)1997年学术出版社。
Abnormalities in the function of oncogenes and tumor suppressor genes have been associated with many human malignancies. The recognition of sites of loss of heterozygosity (LOH) has led to the identification of such genes, We previously reported that abnormalities of mRNA expression of ERCC1 and ERCC2 may be characteristic of epithelial ovarian carcinoma and brain tumors, This led to an investigation of chromosome 19q13.2-q13.4 which contains these DNA repair genes, A 7-Mb region was analyzed using six microsatellite repeats, Loss of heterozygosity has been identified in 53% (8/15) of cases at marker D19S246 which lies in a 2-Mb segment between HRC and KLK1, The genetic material both centromeric and telomeric to the region of loss was conserved, This area is telomeric to three DNA repair genes where LIG1 is 1-Mb centromeric and ERCC1 and ERCC2 are 3.5- and 4.0-Mb centromeric, respectively, These findings represent the first report of a biologically significant rate of LOH on chromosome 19q13.2-q13.4 in human ovarian carcinoma. (C) 1997 Academic Press.