Fluorescence-based sensor membrane for mercury(II) detection
Fluorescence-based sensor membrane for mercury(II) detection
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DOI：
10.1016/s0925-4005(97)80212-2
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发表时间：
1997-03-01
影响因子：
8.4
通讯作者：
Wolfbeis, OS
中科院分区：
文献类型：
--
作者：
Murkovic, I;Wolfbeis, OS
提出了一种用于汞（II）（Hg（II））检测的新型光学传感器膜。传感层由增塑聚氯乙烯（PVC）组成，其中含有亲脂性硼酸盐作为Hg（II）试剂，两亲性氧杂碳菁染料（DiOC（16）（3））作为光学传感器。的传感方案是基于在汞（II）的存在下的硼酸根阴离子的分解。由于这种分解不能用光学方法监测，硼酸根阴离子与荧光氧碳菁阳离子偶联形成亲脂性离子对。硼酸根阴离子的分解导致非荧光染料聚集体的形成，其可以通过荧光测定法进行跟踪。汞（II）从水相到膜的提取导致荧光强度的连续和不可逆的减少，因此动力学方法用于校准。30分钟的暴露时间允许测定100 nM浓度水平的Hg（II）。膜响应Hg（II）与其他金属离子具有非常高的选择性，和pH值的影响被认为是可以忽略不计的。膜可以通过将其暴露于含有相同硼酸盐的溶液中来再生。报道了取代四苯基硼酸盐对膜响应的影响。
A novel optical sensor membrane for the detection of mercury(II) (Hg(II)) is presented. The sensing layer is composed of plasticized poly(vinylchloride) (PVC) containing a lipophilic borate salt as a reagent for Hg(II), and the amphiphilic oxacarbocyanine dye (DiOC(16) (3)) as the optical transducer. The sensing scheme is based on the decomposition of the borate anion in the presence of Hg(II). Since this decomposition cannot be monitored optically, the borate anion is coupled to a fluorescent oxacarbocyanine cation to form a lipophilic ion pair. The decomposition of the borate anion leads to the formation of non-fluorescent dye aggregates which can be followed fluorometrically. The extraction of Hg(II) from the water phase into the membrane leads to a continuous and irreversible decrease in fluorescence intensity, so that a kinetic approach is used for calibration. An exposure time of 30 min allows for the determination of Hg(II) at 100 nM concentration levels. The membranes respond to Hg(II) with very high selectivity over other metal ions, and the pH effects are found to be negligible. The membrane can be regenerated by exposing it to a solution containing the same borate. Effects of substituted tetraphenylborates on the response of the membranes are reported.