Immunocytochemical analysis of beta (β) keratins in the epidermis of chelonians, lepidosaurians, and archosaurians
Immunocytochemical analysis of beta (β) keratins in the epidermis of chelonians, lepidosaurians, and archosaurians
复制标题
DOI：
10.1002/jez.10145
复制
发表时间：
2002-06-15
影响因子：
--
通讯作者：
Sawyer, RH
中科院分区：
文献类型：
--
作者：
Alibardi, L;Sawyer, RH
β角蛋白仅存在于鸟类和爬行动物的表皮中。虽然我们对鸟类β角蛋白的生物化学和分子生物学了解很多，但对爬行动物却知之甚少。在这项研究中，我们已经检查了β角蛋白的分布在海龟，蜥蜴，蛇，鳄蜥，和鳄鱼的成人表皮使用光和电子免疫细胞化学与一个良好的特性的抗血清（抗β（1）抗血清）对已知的鸟类鳞片型β角蛋白。在蜥蜴、蛇和蜥蜴的表皮中，这种抗血清与β层反应强烈，在与β层融合之前与oberhautchen层反应较弱，与中间层反应最弱。此外，抗β（1）抗血清与壁虎的攀缘垫刚毛、海龟的胸甲和甲壳以及鳄鱼表皮的角质层发生特异性反应。电子显微镜研究证实，抗β（1）抗血清仅与形成β层细胞中的特征性3 nm β角蛋白细丝束反应，并与成熟β层中β角蛋白的密集电子透明区域反应。这些免疫细胞化学的结果表明，3纳米β角蛋白丝的爬行动物的外皮是endogenetically相关的那些发现在鸟类的表皮附属物。(C)2002 Wiley-Liss，Inc.
Beta (beta) keratins are present only in the avian and reptilian epidermises. Although much is known about the biochemistry and molecular biology of the beta keratins in birds, little is known for reptiles. In this study we have examined the distribution of beta keratins in the adult epidermis of turtle, lizard, snake, tuatara, and alligator using light and electron immunocytochemistry with a well-characterized antiserum (anti-beta(1) antiserum) made against a known avian scale type beta keratin. In lizard, snake, and tuatara epidermis this antiserum reacts strongly with the beta-layer, more weakly with the oberhautchen before it merges with the beta-layer, and least intensely with the mesos layer. In addition, the anti-beta(1) antiserum reacts specifically with the setae of climbing pads in gekos, the plastron and carapace of turtles, and the stratum corneum of alligator epidermis. Electron microscopic studies confirm that the reaction of the anti-beta(1) antiserum is exclusively with characteristic bundles of the 3-nm beta keratin filaments in the cells of the forming beta-layer, and with the densely packed electron-lucent areas of beta keratin in the mature beta layer. These immunocytochemical results suggest that the 3-nm beta keratin filaments of the reptilian integument are phylogenetically related to those found in avian epidermal appendages. (C) 2002 Wiley-Liss, Inc.