Phycocyanin Improves Reproductive Ability in Obese Female Mice by Restoring Ovary and Oocyte Quality.
Phycocyanin Improves Reproductive Ability in Obese Female Mice by Restoring Ovary and Oocyte Quality.
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藻蓝蛋白通过恢复卵巢和卵母细胞质量来提高肥胖雌性小鼠的生殖能力
DOI：
10.3389/fcell.2020.595373
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发表时间：
2020
影响因子：
5.5
通讯作者：
Liang CG
中科院分区：
文献类型：
--
作者：
Wen X;Han Z;Liu SJ;Hao X;Zhang XJ;Wang XY;Zhou CJ;Ma YZ;Liang CG
与肥胖有关的生殖功能障碍在育龄妇女中日益增加。新出现的证据表明，母体肥胖损害胚胎发育和后代健康，这些缺陷与卵巢和卵母细胞中的氧化应激有关。藻蓝蛋白（PC）是螺旋藻中的一种胆蛋白，具有抗氧化、抗炎和清除自由基的特性。我们前期的研究表明，PC可以减少D-半乳糖致衰老小鼠卵母细胞中活性氧（ROS）的积累。在这里，在癌症研究所（ICR），用高脂饮食（HFD）喂养的小鼠来模拟肥胖，用于测试PC对逆转肥胖引起的生育能力下降的影响。我们观察到一个显着增加的窝仔数和后代的存活率后，PC管理肥胖小鼠。此外，我们发现PC不仅改善了卵巢抗氧化酶的水平，而且还减少了肥胖雌性小鼠卵泡闭锁的发生。此外，卵母细胞中纺锤体-染色体复合体（SCC）的异常形态和异常的线粒体分布模式均得到恢复。PC可部分逆转肥胖相关的ROS积累、早期凋亡细胞数量增加以及卵母细胞中H3 K9 me 3的异常表达。总之，这是第一次证明PC可以通过部分提高肥胖雌性小鼠的卵巢和卵母细胞质量来改善生育能力，并为临床治疗肥胖相关的女性不孕症提供了一种新的策略。
Reproductive dysfunction associated with obesity is increasing among women of childbearing age. Emerging evidence indicates that maternal obesity impairs embryo development and offspring health, and these defects are linked to oxidative stress in the ovary and in oocytes. Phycocyanin (PC) is a biliprotein from Spirulina platensis that possesses antioxidant, anti-inflammatory, and radical-scavenging properties. Our previous studies have shown that PC can reduce reactive oxygen species (ROS) accumulation in oocytes in D-gal-induced aging mice. Here, at the Institute of Cancer Research (ICR) mice fed a high-fat diet (HFD) to model obesity were used to test the effect of PC on reversing the fertility decline caused by obesity. We observed a significant increase in litter size and offspring survival rates after PC administration to obese mice. Further, we found that PC not only ameliorated the level of ovarian antioxidant enzymes, but also reduced the occurrence of follicular atresia in obese female mice. In addition, the abnormal morphology of the spindle-chromosome complex (SCC), and the abnormal mitochondrial distribution pattern in oocytes both recovered. The obesity-related accumulation of ROS, increased number of early apoptotic cells, and the abnormal expression of H3K9me3 in oocytes were all partially reversed after PC administration. In summary, this is the first demonstration that PC can improve fertility by partially increasing ovarian and oocyte quality in obese female mice and provides a new strategy for clinically treating obesity-related infertility in females.