GENETICS OF SPONTANEOUS, HIGH-FREQUENCY LOSS OF B-CAPSULE EXPRESSION IN HEMOPHILUS-INFLUENZAE
GENETICS OF SPONTANEOUS, HIGH-FREQUENCY LOSS OF B-CAPSULE EXPRESSION IN HEMOPHILUS-INFLUENZAE
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DOI：
10.1128/iai.49.2.389-395.1985
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
MOXON, ER
中科院分区：
文献类型：
--
作者：
HOISETH, SK;CONNELLY, CJ;MOXON, ER
The frequency of spontaneous capsule loss in H. influenza type b is shown to be 0.1-0.3%. All of 10 independent capsule-deficient variants (derived from 4 different type b strains) were found to be missing an identical 9-kilobase EcoRI restiction fragment when probed with a cloned mice of DNA containing sequence known to be necessary for type b capsule expression. These results suggest the existence of a specific mechanism for shutting of type b capsule synthesis at a high frequency. Intranasal infection of infant rats showed that capsule loss occurred in vivo at frequencies comparable to those observed in vitro.
