Abeta(1-40) forms five distinct amyloid structures whose beta-sheet contents and fibril stabilities are correlated.
Abeta(1-40) forms five distinct amyloid structures whose beta-sheet contents and fibril stabilities are correlated.
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DOI：
10.1016/j.jmb.2010.06.023
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发表时间：
2010-08-20
影响因子：
5.6
通讯作者：
Wetzel R
中科院分区：
文献类型：
--
作者：
Kodali R;Williams AD;Chemuru S;Wetzel R
The ability of a single polypeptide sequence to grow into multiple stable amyloid fibrils sets these aggregates apart from most native globular proteins. The existence of multiple amyloid forms is the basis for strain effects in yeast prion biology, and may also contribute to variations in Alzheimer’s disease pathology. However, the structural basis for amyloid polymorphism is poorly understood. We report here five structurally distinct fibrillar aggregates of the Alzheimer’s plaque peptide Aβ(1–40), as well as a non-fibrillar aggregate induced by Zn+2. Each of these conformational forms exhibits a unique profile of physical properties, and all the fibrillar forms “breed true” in elongation reactions at a common set of growth conditions. Consistent with their defining cross-β structure, we find that in this series the amyloid fibrils containing more extensive β-sheet exhibit greater stability. At the same time, side chain packing outside of the β-sheet regions also contributes to stability, and to stability differences between polymorphic forms. Stability comparison is facilitated by the unique feature that the free energy of the monomer (equivalent to the unfolded state in a protein folding reaction) does not vary, and hence can be ignored, in the comparison of ΔG° of elongation values for each polymorphic fibril obtained at a single set of conditions.
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DOI：
10.1016/j.bbapap.2010.04.001
发表时间：
2010-07
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Biancalana M;Koide S
通讯作者：
Koide S
影响因子：
64.8
作者：
GRIFFITH, JS
通讯作者：
GRIFFITH, JS
DOI：
10.1073/pnas.0705888104
发表时间：
2007-10-02
影响因子：
11.1
作者：
Gardberg, Anna S.;Dice, Lezlee T.;Dealwis, Chris
通讯作者：
Dealwis, Chris
影响因子：
8
作者：
Kheterpal, I;Wetzel, R;Cook, KD
通讯作者：
Cook, KD
影响因子：
5.6
作者：
Jahn, Thomas R.;Makin, O. Sumner;Serpell, Louise C.
通讯作者：
Serpell, Louise C.