Peristalsis in the junction region of the Drosophila larval midgut is modulated by DH31 expressing enteroendocrine cells.
Peristalsis in the junction region of the Drosophila larval midgut is modulated by DH31 expressing enteroendocrine cells.
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DOI：
10.1186/1472-6793-10-14
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发表时间：
2010-08-10
期刊：
影响因子：
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通讯作者：
Zapotoczny G
中科院分区：
文献类型：
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作者：
LaJeunesse DR;Johnson B;Presnell JS;Catignas KK;Zapotoczny G
协调消化生理过程的潜在细胞和分子机制是复杂、神秘的，涉及多个细胞和器官系统的整合。在所有肠道中，肠道的蠕动作用将食物从前端移动到后端，通过消化的各个阶段，其流动速度取决于肠道本身的内在和外在信号。我们已经确定了一种调节黑腹果蝇幼虫中肠肠道运动的肠内分泌细胞类型。这些细胞位于前肠和中肠酸性部分的交界处，是一组肠内分泌细胞，在肠道这一区域表达肽激素利尿激素31。通过细胞消融和通过表达莱茵衣藻蓝光激活通道蛋白的异位激活，我们证明了这些肠内分泌细胞对于中肠连接区域的蠕动既是必要的也是充分的，并且需要利尿激素31来影响该区域的正常蠕动。在中肠的同一连接区域内，我们还发现了形态学特征，表明该区域起着调节食物从前中肠进入肠道酸性部分的阀门的作用。我们描述了一组被称为中肠连接DH31的肠内分泌细胞，这些细胞表达了果蝇幼虫中肠前部和酸性区域之间连接区域的蠕动运动所必需的细胞。我们已经证明，表达DH31的细胞对于运动是必要的和充分的，DH31肽激素对于中肠连接区域的蠕动是必需的。果蝇模型系统将允许对消化过程进行进一步的解剖，并提供对所有生物体中调节消化的机制的更好理解。
The underlying cellular and molecular mechanisms that coordinate the physiological processes in digestion are complex, cryptic, and involve the integration of multiple cellular and organ systems. In all intestines, peristaltic action of the gut moves food through the various stages of digestion from the anterior end towards the posterior, with the rate of flow dependent on signals, both intrinsic and extrinsic to the gut itself. We have identified an enteroendocrine cell type that regulates gut motility in the Drosophila melanogaster larval midgut. These cells are located at the junction of the anterior and the acidic portions of the midgut and are a group of enteroendocrine cells that express the peptide hormone Diuretic Hormone 31 in this region of the gut. Using cell ablation and ectopic activation via expression of the Chlamydomonas reinhardtii blue light-activated channelopsin, we demonstrate that these enteroendocrine cells are both necessary and sufficient for the peristalsis in the junction region of the midgut and require the Diuretic Hormone 31 to affect normal peristalsis in this region. Within the same junction region of the midgut, we have also identified morphological features suggesting that this region acts as a valve that regulates the transit of food from the anterior midgut into the acidic portion of the gut. We have characterized and described a set of enteroendocrine cells called the Midgut Junction DH31 expressing cells that are required for peristaltic movement in the junction region between the anterior portion and acidic region of the larval midgut of Drosophila melanogaster. We have shown that the Midgut Junction DH31 expressing cells are necessary and sufficient for motility and that the peptide hormone DH31 is required for peristalsis in the junction region of the midgut. The Drosophila model system will allow for a further dissection of the digestion process and provide a better understanding of the mechanisms that regulate digestion in all organisms.