Characterization of Protein Alterations in Damaged Axons in the Brainstem Following Traumatic Brain Injury Using Fourier Transform Infrared Microspectroscopy: A Preliminary Study
Characterization of Protein Alterations in Damaged Axons in the Brainstem Following Traumatic Brain Injury Using Fourier Transform Infrared Microspectroscopy: A Preliminary Study
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DOI：
10.1111/1556-4029.12743
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发表时间：
2015-05
影响因子：
1.6
通讯作者：
Ji Zhang;F. Niu;Hongmei Dong;Liang Liu;J. Li;Shangxun Li
中科院分区：
文献类型：
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作者：
Ji Zhang;F. Niu;Hongmei Dong;Liang Liu;J. Li;Shangxun Li
轴突损伤是脑外伤后神经功能障碍的重要原因，但目前的组织学诊断方法在确定损伤轴突的病理特征方面存在局限性，无法提供客观准确的定量。傅里叶变换红外显微光谱（FTIRM）技术通过计算谱带吸收强度，能够提供生物组织的大分子生物信息，包括生物化学组成和结构。本研究采用傅里叶变换红外反射显微镜（FTIRM）技术，对创伤性脑损伤大鼠伤后72 h脑干轴突损伤进行检测，并经β-淀粉样前体蛋白（β-APP）免疫染色证实。酰胺I带下的红外吸收的较低强度强烈对应于轴突损伤的区域，并且酰胺I带的进一步分析显示损伤和正常轴突之间的蛋白质构象的显著差异。研究结果表明，应用FTIR技术，酰胺I带有可能成为轴索损伤的红外光谱标记。
Axonal injury contributes greatly to neurological dysfunction following traumatic brain injury (TBI), but current histological diagnostic methods are limited in identifying the pathological profiles of injured axons and unable to provide an objective and accurate quantification. Fourier transform infrared microspectroscopy (FTIRM) has the ability to offer macromolecular bioinformatics of the tissues including biochemical composition and structure by calculating band absorption intensity. In this study, axonal injury in the brainstem of rats with traumatic brain injury at 72 h post‐trauma, which was confirmed with beta‐amyloid precursor protein (β‐APP) immunostaining, was detected with FTIRM technique. The lower intensity of infrared absorbance under the amide I band corresponds strongly to the area of axonal injury, and further analysis of amide I band shows significant differences in protein conformation between injured and normal axons. The findings indicate that using FTIRM technique, the amide I band has potentials to be a infrared spectral marker of axonal injury.