Soybean maturity genes associated with seed coat pigmentation and cracking in response to low temperatures
Soybean maturity genes associated with seed coat pigmentation and cracking in response to low temperatures
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DOI：
10.2135/cropsci1999.3961657x
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发表时间：
1999-11-01
期刊：
影响因子：
2.3
通讯作者：
Abe, J
中科院分区：
文献类型：
--
作者：
Takahashi, R;Abe, J
大豆[Glycine max（L.）梅尔。]在开花时，将种子置于低温（约15 ℃）下，会导致种脐周围的布朗宁和种子开裂。色素沉着和裂纹都降低了大豆种子的外观，降低了它们的商业价值。早期的一项研究表明，负责色素沉着的基因之一与成熟基因密切相关。本研究的目的是评估五个大豆成熟度基因（E-1-E-5）对种皮着色和开裂强度的影响。大豆品种Harosoy（e（1）e（2）E（3）E（4）e（5））及其E1至Eg基因座的近等基因系（NIL）从开花后8d开始在15 ℃下暴露2周。对照植物在其整个生命周期中在温室中生长，而处理的植物从温室转移到人工气候室进行冷冻处理。色素沉着的强度不受E（3）的影响，E-2和E（4）略微降低，E-1和E-5显著降低。e（3）使开裂程度略有增加，e（4）、E-1和E-5使开裂程度急剧降低。这些结果表明，大豆成熟度基因中的一些基因对低温胁迫下种皮着色和开裂的强度具有抑制作用。显性等位基因El和ES在抑制色素沉着和开裂方面最有效。因此，这两个基因可能是有用的，以确保耐冷胁迫的品种与e（3）和e（4），共同条件下的长日照不敏感，在高纬度地区的适应性性状。
Exposure of soybean [Glycine max (L.) Merr.] to chilling temperatures (approximate to 15 degrees C) at flowering induces browning around the hilum region and cracking of the seed feats. Both pigmentation and cracking degrade the external appearance of soybean seeds and reduce their commercial value. An earlier study showed that one of the genes responsible for pigmentation is closely associated with a maturity gene. The objective of this study was to evaluate the effect of five soybean maturity genes (E-1-E-5) on the intensity of seed coat pigmentation and cracking. Soybean cv. Harosoy (e(1)e(2)E(3)E(4)e(5)) and its near-isogenic lines (NIL) for El to Eg loci were exposed to 15 degrees C for 2 wk beginning 8 d after anthesis. Control plants were grown in a greenhouse throughout their life cycle, whereas treated plants were transferred from the greenhouse to a phytotron for the chilling treatment. Intensity of pigmentation was not affected by e(3), slightly reduced by E-2 and e(4), and profoundly reduced by E-1 and E-5. Degree of cracking was slightly increased by e(3) and drastically reduced by e(4), E-1, and E-5. The results suggest that some of the soybean maturity genes have inhibitory effects on the intensity of seed coat pigmentation and cracking in response to low temperatures. Dominant alleles El and ES are most effective in suppressing both pigmentation and cracking. Therefore, these two genes may be useful to ensure tolerance to chilling stress in cultivars with e(3) and e(4), which jointly condition the insensitivity to long daylength, an adaptive trait in high latitude regions.