Mesenchymal stem cell adhesion but not plasticity is affected by high substrate stiffness.
Mesenchymal stem cell adhesion but not plasticity is affected by high substrate stiffness.
复制标题
高基质硬度影响间充质干细胞粘附，但不影响可塑性。
DOI：
10.1088/1468-6996/13/6/064205
复制
发表时间：
2012
影响因子：
5.5
通讯作者：
Tam KV
中科院分区：
文献类型：
--
作者：
Tam KV
无论生物供应如何，合成材料都具有诱导细胞特异性反应的公认能力，这为组织工程师提供了寻找合适的材料和条件来制备组织靶向支架的机会。干细胞和成熟细胞已被证明在体外获得不同的形态，并在具有可调机械性能的合成材料上生长时改变其表型。用于细胞培养的基质的硬度可能为细胞提供模仿给定生理或病理条件的机械线索，从而影响细胞的生物学特性。细胞对基质成分和机械性能的敏感性存在于称为粘着斑的多蛋白复合物中，其动态修饰导致细胞骨架重塑和基因表达的变化。在这项研究中，在细胞-基质相互作用的第一阶段，使用具有相似化学成分和不同弹性的聚ε-己内酯平面膜，追踪人类间充质干细胞中粘着斑对基质硬度的响应。与成熟的真皮成纤维细胞相比，间充质干细胞在粘附方面表现出对基质硬度的特异性反应，这是差异粘着斑组装的结果，而它们作为整体的多能性并未受到基质顺应性的显着影响。鉴于干细胞对基质力学的敏感性，在制备组织特异性支架之前，需要对此类细胞的力学生物学进行进一步研究。
The acknowledged ability of synthetic materials to induce cell-specific responses regardless of biological supplies provides tissue engineers with the opportunity to find the appropriate materials and conditions to prepare tissue-targeted scaffolds. Stem and mature cells have been shown to acquire distinct morphologies in vitro and to modify their phenotype when grown on synthetic materials with tunable mechanical properties. The stiffness of the substrate used for cell culture is likely to provide cells with mechanical cues mimicking given physiological or pathological conditions, thus affecting the biological properties of cells. The sensitivity of cells to substrate composition and mechanical properties resides in multiprotein complexes called focal adhesions, whose dynamic modification leads to cytoskeleton remodeling and changes in gene expression. In this study, the remodeling of focal adhesions in human mesenchymal stem cells in response to substrate stiffness was followed in the first phases of cell–matrix interaction, using poly-ε-caprolactone planar films with similar chemical composition and different elasticity. As compared to mature dermal fibroblasts, mesenchymal stem cells showed a specific response to substrate stiffness, in terms of adhesion, as a result of differential focal adhesion assembly, while their multipotency as a bulk was not significantly affected by matrix compliance. Given the sensitivity of stem cells to matrix mechanics, the mechanobiology of such cells requires further investigations before preparing tissue-specific scaffolds.