Hydroxyapatite growth induced by native extracellular matrix deposition on solid surfaces.
Hydroxyapatite growth induced by native extracellular matrix deposition on solid surfaces.
复制标题
由固体表面上的天然细胞外基质沉积诱导羟基磷灰石生长。
DOI：
10.22203/ecm.v009a02
复制
发表时间：
2005
影响因子：
3.1
通讯作者：
M. Stutzmann
中科院分区：
文献类型：
--
作者：
L. Pramatarova;E. Pecheva;R. Presker;M. Pham;M. Maitz;M. Stutzmann
生物系统具有非凡的能力，可以产生完美的精细结构，如贝壳，珍珠，骨骼，牙齿和珊瑚。这些结构是相互作用的无机（磷酸钙或碳酸盐矿物）和有机对应物的复合物。很难确定哪一部分起主要作用。例如，软体动物壳晶体的生长被认为是由细胞外有机基质（ECM）从溶液中引发的。根据该理论，基质诱导含钙晶体的成核。最近，另一种理论已经提出，指出一类粒细胞血细胞将直接参与牡蛎壳晶体的生产。在这里介绍的工作中，AISI 316不锈钢的表面通过ECM蛋白的沉积进行改性。通过两种方法研究了改性基质诱导羟基磷灰石（HA）从模拟体液（SBF）中成核和生长的能力：（1）在SBF中的简单浸泡过程和（2）激光-液-固相互作用（LLSI）过程，该过程允许扫描激光束与浸没在SBF中的固体基质之间的相互作用。通过傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）对沉积的HA层进行了研究。结果发现，与天然ECM蛋白质的不锈钢表面的涂层诱导HA的成核和生长，并促进其结晶。通过简单浸泡样品的过程，无论其在溶液中的水平或垂直位置，由于反应性ECM涂覆的不锈钢表面，HA层生长。结果表明，在生长的第一阶段发生的过程不仅是重力的结果。LLSI工艺的应用通过促进和增强HA成核和生长而强烈影响ECM改性基底上的HA形成，所述促进和增强HA成核和生长是通过几种刺激的协同效应实现的，即，改性固体表面、激光束和水溶液。
Biological systems have a remarkable capability to produce perfect fine structures such as seashells, pearls, bones, teeth and corals. These structures are composites of interacting inorganic (calcium phosphate or carbonate minerals) and organic counterparts. It is difficult to say with certainty which part has the primary role. For example, the growth of molluscan shell crystals is thought to be initiated from a solution by the extracellular organic matrix (ECM). According to this theory, the matrix induces nucleation of calcium containing crystals. Recently, an alternative theory has been put forward, stating that a class of granulocytic hemocytes would be directly involved in shell crystal production in oysters. In the work presented here the surface of AISI 316 stainless steel was modified by deposition of ECM proteins. The ability of the modified substrates to induce nucleation and growth of hydroxyapatite (HA) from simulated body fluid (SBF) was examined by a kinetic study using two methods: (1) a simple soaking process in SBF and (2) a laser-liquid-solid interaction (LLSI) process which allows interaction between a scanning laser beam and a solid substrate immersed in SBF. The deposited HA layers were investigated by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). It was found that a coating of stainless steel surface with native ECM proteins induced nucleation and growth of HA and facilitated its crystallization. By the process of simple soaking of the samples, irrespective of their horizontal or vertical position in the solution, HA layers were grown due to the reactive ECM-coated stainless steel surface. It was shown that the process occurring in the first stages of the growth was not only a result of the force of gravity. The application of the LLSI process strongly influenced HA formation on the ECM-modified substrates by promoting and enhancing the HA nucleation and growth through a synergistic effect of a few stimuli, i.e., the modified solid surface, the laser beam and the aqueous solution.