CADHERIN-11 EXPRESSED IN ASSOCIATION WITH MESENCHYMAL MORPHOGENESIS IN THE HEAD, SOMITE, AND LIMB BUD OF EARLY MOUSE EMBRYOS
CADHERIN-11 EXPRESSED IN ASSOCIATION WITH MESENCHYMAL MORPHOGENESIS IN THE HEAD, SOMITE, AND LIMB BUD OF EARLY MOUSE EMBRYOS
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DOI：
10.1006/dbio.1995.1149
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发表时间：
1995-05-01
影响因子：
2.7
通讯作者：
TAKEICHI, M
中科院分区：
文献类型：
--
作者：
KIMURA, Y;MATSUNAMI, H;TAKEICHI, M
钙粘蛋白-11（cad-11）是最近通过聚合酶链反应鉴定的细胞粘附分子钙粘蛋白家族的新成员。为了研究该分子的功能和表达，我们克隆了小鼠cad-11的cDNA。用cDNA转染L细胞导致它们获得典型的钙粘蛋白依赖性细胞-细胞粘附，并且表达cad-11的L细胞在与表达E-、P-、N-或R-钙粘蛋白的L细胞混合时不共聚集，这表明这种新型钙粘蛋白具有同嗜性结合特异性，如对其他钙粘蛋白所发现的。为了确定该分子的发育表达模式，我们对早期小鼠胚胎进行了原位杂交分析。Cad-11在原始条纹中期至晚期仅在头部和尾部区域的中胚层层中首次出现。在头部，这种外观随后在包括鳃弓的间充质组织中强烈表达。在树干中，近轴中胚层最初不表达cad-11。然而，随着体节的形成，它们表达cad-11，并且这种表达与它们最初的凝聚和从有丝分裂前中胚层分离密切相关。cad-11在体节中的表达最终仅限于硬节细胞。随着肢芽的发育，cad-11出现在肢间充质的远端部分，在后期，其表达在外周间充质中最为明显。因此，Cad-11在早期胚胎中由有限的间充质细胞群表达，尽管它也在神经管的部分区域表达，如视泡和背中线，以及部分耳泡。作为研究cad-11在间充质细胞粘附中作用的一步，我们将肢芽间充质分离成单个细胞，沉淀它们，并将它们培养为聚集体。在这些培养物中，cad-11阳性细胞明显地从阴性细胞群中分离出来，这表明cad-11可能参与了间充质细胞的选择性结合。为了比较，我们研究了N-cadherin的表达，发现这两种cadherin的表达是有差异的，并且在某些组织中是互补的。这些结果表明，cad-11参与了胚胎早期间充质细胞亚群和某些神经细胞的特异性结合。(C)出版社：Academic Press
Cadherin-11 (cad-11) is a novel member of the cadherin family of cell adhesion molecules, having recently been identified by means of the polymerase chain reaction. To study the function and expression of this molecule, we cloned mouse cad-11 cDNA. Transfection of L cells with cDNA led them to acquire a typical cadherin-dependent cell-cell adhesiveness, and the L cells expressing cad-11 did not coaggregate with L cells expressing E-, P-, N-, or R-cadherin when they were mixed, indicating that this novel cadherin has a homophilic binding specificity, as found for other cadherins. To determine the developmental expression pattern of this molecule, we performed in situ hybridization analysis on early mouse embryos. Cad-11 first appeared in mesodermal layers only in the head and tail regions at the mid-to-late primitive streak stages. In the head, this appearance was followed by strong expression in mesenchymal tissues including branchial arches. In the trunk, the paraxial mesoderm initially did not express cad-11. However, as the somites formed, they expressed cad-11, and this expression was strictly correlated with their initial condensation and segregation from the presomitic mesoderm. The cad-11 expression in the somites was eventually restricted to sclerotome cells. As the limb buds developed, cad-11 appeared in the distal portion of the limb mesenchyme, and, at later stages, its expression was most evident at the peripheral mesenchyme. Cad-11 was thus expressed by restricted populations of mesenchymal cells in early embryos, although it was also expressed in parts of the neural tube, such as the optic vesicle and dorsal midline, and in part of the otic vesicle. As a step to investigate the role of cad-11 in mesenchymal cell adhesion, we dissociated the limb bud mesenchyme into single cells, pelleted them, and cultured them as aggregates. In these cultures, cad-11-positive cells clearly sorted out of the negative cell population, suggesting that cad-11 might be involved in selective association of mesenchymal cells. For comparison, we studied the expression of N-cadherin and found that the expressions of these two cadherins were differential, and complementary in some tissues. These results suggest that cad-11 is involved in specific associations of subsets of mesenchymal cells and also of some neural cells during early embryogenesis. (C) 1995 Academic Press, Inc.