Mussel-inspired bioceramics with self-assembled Ca-P/polydopamine composite nanolayer: Preparation, formation mechanism, improved cellular bioactivity and osteogenic differentiation of bone marrow stromal cells
Mussel-inspired bioceramics with self-assembled Ca-P/polydopamine composite nanolayer: Preparation, formation mechanism, improved cellular bioactivity and osteogenic differentiation of bone marrow stromal cells
复制标题
具有自组装Ca-P/聚多巴胺复合纳米层的贻贝生物陶瓷：制备、形成机制、改善骨髓基质细胞的细胞生物活性和成骨分化
DOI：
10.1016/j.actbio.2013.10.013
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发表时间：
2014
影响因子：
9.7
通讯作者：
Xiao Yin
中科院分区：
文献类型：
--
作者：
Wu Chengtie;Han Pingping;Liu Xiaoguo;Xu Mengchi;Tian Tian;Chang Jiang;Xiao Yin
纳米结构的生物材料表面在提高其体外细胞活性和促进体内组织再生方面起着重要作用。受贻贝粘附多功能性的启发，这被认为是由于斑块-底物界面富含3,4-二羟基-l-苯三胺（DOPA）和赖氨酸氨基酸，在本研究中，我们开发了一种自组装方法，通过将β-TCP生物陶瓷浸泡在tris -多巴胺溶液中，在β-三磷酸钙（β-TCP）表面制备均匀的磷酸钙(Ca-P)/聚多巴胺复合纳米层。结果表明，多巴胺的加入量、反应温度和反应时间是诱导形成均匀的Ca-P/聚多巴胺复合纳米层的三个关键因素。Ca-P/聚多巴胺复合纳米层的形成机制包括两个重要步骤：(i)在Tris-HCl溶液中加入多巴胺降低了pH值，加速了Ca和P离子从β-TCP陶瓷晶界的溶解；（ii）多巴胺聚合形成自组装的聚多巴胺膜，同时在聚多巴胺的帮助下矿化纳米级Ca-P颗粒，其中聚多巴胺的形成与Ca-P矿化同时发生（形成由磷酸钙基材料组成的纳米级微颗粒），最终在β-TCP陶瓷表面形成自组装的Ca-P/聚多巴胺复合纳米层。此外，形成的自组装Ca-P/聚多巴胺复合纳米层显著提高了β-TCP陶瓷的表面粗糙度和亲水性，促进了人骨髓基质细胞的附着、增殖、碱性磷酸盐（ALP）活性和骨相关基因（ALP、OCN、COL1和Runx2）的表达。我们的研究结果表明，制备自组装Ca-P/聚多巴胺复合纳米层是一种可行的方法来修饰生物材料的表面，显著改善其表面物理化学性质和细胞生物活性，用于骨再生应用。
The nanostructured surface of biomaterials plays an important role in improving their in vitro cellular bioactivity as well as stimulating in vivo tissue regeneration. Inspired by the mussel’s adhesive versatility, which is thought to be due to the plaque–substrate interface being rich in 3,4-dihydroxy-l-phenylalamine (DOPA) and lysine amino acids, in this study we developed a self-assembly method to prepare a uniform calcium phosphate (Ca-P)/polydopamine composite nanolayer on the surface of β-tricalcium phosphate (β-TCP) bioceramics by soaking β-TCP bioceramics in Tris–dopamine solution. It was found that the addition of dopamine, reaction temperature and reaction time are three key factors inducing the formation of a uniform Ca-P/polydopamine composite nanolayer. The formation mechanism of a Ca-P/polydopamine composite nanolayer involved two important steps: (i) the addition of dopamine to Tris–HCl solution decreases the pH value and accelerates Ca and P ionic dissolution from the crystal boundaries of β-TCP ceramics; (ii) dopamine is polymerized to form self-assembled polydopamine film and, at the same time, nanosized Ca-P particles are mineralized with the assistance of polydopamine, in which the formation of polydopamine occurs simultaneously with Ca-P mineralization (formation of nanosized microparticles composed of calcium phosphate-based materials), and finally a self-assembled Ca-P/polydopamine composite nanolayer forms on the surface of the β-TCP ceramics. Furthermore, the formed self-assembled Ca-P/polydopamine composite nanolayer significantly enhances the surface roughness and hydrophilicity of β-TCP ceramics, and stimulates the attachment, proliferation, alkaline phosphate (ALP) activity and bone-related gene expression (ALP, OCN, COL1 and Runx2) of human bone marrow stromal cells. Our results suggest that the preparation of self-assembled Ca-P/polydopamine composite nanolayers is a viable method to modify the surface of biomaterials by significantly improving their surface physicochemical properties and cellular bioactivity for bone regeneration application.