Neural precursor cells derived from induced pluripotent stem cells exhibit reduced susceptibility to infection with a neurotropic coronavirus.
Neural precursor cells derived from induced pluripotent stem cells exhibit reduced susceptibility to infection with a neurotropic coronavirus.
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来自诱导多能干细胞的神经前体细胞表现出对嗜神经冠状病毒感染的易感性降低。
DOI：
10.1016/j.virol.2017.08.003
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Lane,ThomasE
中科院分区：
文献类型：
--
作者：
Mangale,Vrushali;Marro,BrettS;Plaisted,WarrenC;Walsh,CraigM;Lane,ThomasE
本研究检测了小鼠诱导的多能干细胞源性神经前体细胞(IPSC-NPC)对嗜神经性JHM株小鼠肝炎病毒(JHMV)感染的敏感性。与出生后第1天绿色荧光蛋白转基因小鼠纹状体来源的神经干细胞类似，IPSC来源的神经干细胞能够分化为终末神经细胞类型，并在干扰素-γ治疗后表达MHC I和II类。然而，与出生后来源的NC相比，IPSC-NC表达低水平的癌胚抗原-细胞黏附分子1a(CEACAM1a)，JHMV的表面受体，并且更不容易受到感染和病毒诱导的细胞病变效应。这在抗病毒感染的鼻咽癌治疗应用方面的相关性进行了讨论。
The present study examines the susceptibility of mouse induced pluripotent stem cell-derived neural precursor cells (iPSC-NPCs) to infection with the neurotropic JHM strain of mouse hepatitis virus (JHMV). Similar to NPCs derived from striatum of day 1 postnatal GFP-transgenic mice (GFP-NPCs), iPSC-derived NPCs (iPSC-NPCs) are able to differentiate into terminal neural cell types and express MHC class I and II in response to IFN-γ treatment. However, in contrast to postnatally-derived NPCs, iPSC-NPCs express low levels of carcinoembryonic antigen-cell adhesion molecule 1a (CEACAM1a), the surface receptor for JHMV, and are less susceptible to infection and virus-induced cytopathic effects. The relevance of this in terms of therapeutic application of NPCs resistant to viral infection is discussed.