In vivo expression of interleukin 5 induces an eosinophilia and expanded Ly-1B lineage populations.
In vivo expression of interleukin 5 induces an eosinophilia and expanded Ly-1B lineage populations.
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白细胞介素 5 的体内表达可诱导嗜酸性粒细胞增多并扩大 Ly-1B 谱系群体。
DOI：
10.1093/intimm/2.10.965
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发表时间：
1990
影响因子：
4.4
通讯作者：
Johnson,GR
中科院分区：
文献类型：
--
作者：
Vaux,DL;Lalor,PA;Cory,S;Johnson,GR
在体外，小鼠白细胞介素5（IL-5）作为嗜酸性粒细胞集落刺激因子，诱导B细胞增殖和分泌抗体。为了研究IL-5在体内的生物学效应，我们将经致死剂量照射的小鼠骨髓细胞与携带IL-5编码序列的重组逆转录病毒一起移植。在2周内，受体小鼠的外周血表现出明显的嗜酸性粒细胞，持续至少12个月，并且在骨髓、脾、肝、肺和肠中也明显存在过量的嗜酸性粒细胞。虽然在常规B淋巴细胞群中未检测到变化，但腹膜充满了Ly-1谱系特征性的B细胞。尽管这些细胞群扩增，小鼠在移植后12个月内保持健康。这些结果表明IL-5主要作用于Ly-1谱系的嗜酸性粒细胞和B细胞，并且这些细胞类型的持续过量产生不是致病性的。
In vitro, murine interieukin 5 (IL-5) acts as a colony-stimulating factor tor eoalnophils and induces B cells to proliferate and secrete antibody. In order to asseas the biological effects of IL-5in vivo, we transplanted lethally irradiated mlce wlth bone marrow cells Infected with a recombinant retrovirus bearing the IL-5 coding sequence. Within 2 weeks the peripheral blood of recipient mlce exhibited a marked eosinophilla which persisted for at least 12 months, and an excess number of eosinophils was also evident In the bone marrow, spleen, liver, lung, and gut. Although no changes could be detected In the conventional B lymphocyte population, the perttoneum was replete wlth B cells characteristic of the Ly-1 lineage. Despite these expanded cell populations, mice remained healthy for 12 months after transplantation. These results suggest that IL-5 acts primarily on eosinophils and B cells of the Ly-1 lineage, and that persistent overproduction of these cell types is not pathogenic.