BASIC FIBROBLAST GROWTH-FACTOR (BFGF) AND 2 OF ITS RECEPTORS, FGFR1 AND FGFR2 - GENE-EXPRESSION IN THE RAT-BRAIN DURING POSTNATAL-DEVELOPMENT AS DETERMINED BY QUANTITATIVE RT-PCR
BASIC FIBROBLAST GROWTH-FACTOR (BFGF) AND 2 OF ITS RECEPTORS, FGFR1 AND FGFR2 - GENE-EXPRESSION IN THE RAT-BRAIN DURING POSTNATAL-DEVELOPMENT AS DETERMINED BY QUANTITATIVE RT-PCR
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DOI：
10.1016/0303-7207(94)90122-8
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发表时间：
1994-09-01
影响因子：
4.1
通讯作者：
SHIU, RPC
中科院分区：
文献类型：
--
作者：
ELHUSSEINI, AE;PATERSON, JA;SHIU, RPC
在雄性大鼠出生后早期发育过程中，检查了碱性成纤维细胞生长因子（bFGF）及其两种高亲和力受体（FGFR1和FGFR2）的区域和时间表达模式；使用逆转录聚合酶链式反应 (RT-PCR) 获得 mRNA 测量值，将其相对于 GAPDH 的 mRNA 表示为常数。在大鼠大脑中，bFGF 和 FGFR2 的 mRNA 数量在出生后第一周内相对较低，但到 28 天时，它们与 1 岁大鼠大脑中的水平一样高。相比之下，FGFR1 的表达是双相的：出生后第 1 天和第 28 天的 mRNA 水平高于第 21 天。对 28 天龄大鼠大脑显微解剖区域的 mRNA 定量显示，bFGF 和 FGFR2 的表达在区域之间显示出显着差异，但 FGFR1 的表达在分析的区域之间似乎变化较小。对于所有这三个基因，海马体似乎都具有相对较高量的 mRNA。在出生后早期发育过程中，bFGE、FGFR1 和 FGFR2 的表达时间模式也因大脑区域的不同而不同。在枕叶皮质和下丘中，bFGF 和 FGFR2 的 mRNA 数量在第一个月内均有所增加，与 FGFR1 不同。然而，在小脑中，bFGF 和 FGFR1 mRNA 的最高表达出现在出生后第 1 天； FGFR2 表达明显随年龄变化较小。出生后早期发育过程中不同脑区 bFGE FGFR1 和 FGFR2 表达的时间变化表明，受体调节可能会根据脑区和发育年龄产生不同的 bFGF 生理效应。
Regional and temporal patterns of the expression of basic fibroblast growth factor (bFGF), and two of its high affinity receptors (FGFR1 and FGFR2), were examined in the male rat brain during early postnatal development; the reverse transcription-polymerase chain reaction (RT-PCR) was used to obtain mRNA measurements which were expressed relative to mRNA for GAPDH as a constant. In the rat cerebrum, the mRNAs for bFGF and for FGFR2 were relatively low in amount within the first postnatal week, but by 28 days, they were as high as in the 1-year-old rat cerebrum. In contrast, the expression of FGFR1 was biphasic: mRNA levels were higher at postnatal days 1 and 28 than at day 21. Quantitation of mRNA from microdissected regions of 28-day-old rat brain revealed that the expression of bFGF and of FGFR2 showed a marked variation between regions but the expression of FGFR1 appeared less variable between the regions that were analyzed. For all three genes the hippocampus appeared to have high relative amounts of mRNA. The temporal patterns of expression of bFGE FGFR1 and FGFR2 also differed with brain region during early postnatal development. In the occipital cortex and inferior colliculus, the mRNAs for bFGF and FGFR2 both increased in amount during the first month, unlike that for FGFR1. However, in the cerebellum, the highest expression of bFGF and FGFR1 mRNAs occurred at postnatal day 1; FGFR2 expression apparently showed less change with age. The temporal changes in bFGE FGFR1 and FGFR2 expression in different brain regions during early postnatal development suggest that receptor regulation may permit different physiological effects of bFGF according to brain region and developmental age.