Detection of Cryptosporidium spp. and Giardia duodenalis in surface water: A health risk for humans and animals
Detection of Cryptosporidium spp. and Giardia duodenalis in surface water: A health risk for humans and animals
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DOI：
10.1016/j.watres.2009.06.020
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发表时间：
2009-09-01
期刊：
影响因子：
12.8
通讯作者：
Mezo, Mercedes
中科院分区：
文献类型：
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作者：
Antonio Castro-Hermida, Jose;Garcia-Presedo, Ignacio;Mezo, Mercedes
本研究的目的是确定隐孢子虫属的污染程度。和贾第鞭毛虫（Giardiacetalalis）。在主要流域（包括5个休闲区）的22个点采集水样（50 L），并在3条最重要的河流的源头和河口以及较小的次级河流的河口。从该地区养殖的18个牛群中随机选择的奶牛中收集粪便样本。共对139头新生小牛、480头小母牛和697头奶牛进行了采样。确定了两种肠道病原体感染的患病率、感染强度和与感染传播相关的风险。在2007年春、夏、秋、冬四季采集水样和粪便样品。鉴定了水样中存在的这些寄生虫的种类和基因型。在水和粪便样品中，春季和夏季比秋季和冬季收集到更多的寄生虫阶段。春季隐孢子虫（Cryptosporidium spp.）在13个牛群（72.2%）的33头（9.4%）奶牛中检出了卵囊;在15个农场（83.3%）的56头（16.0%）奶牛中检测到尿道囊肿，感染强度范围为5 - 7895 G。每克粪便中的尿道囊肿数。隐孢子虫的感染阶段。和G.春季分别有26份（89.6%）和27份（93.1%）水样检出铜绿假单胞菌。寄生虫的平均浓度范围为2至1200隐孢子虫属。卵囊数为2 ~ 400 G.尿道囊肿每升。隐孢子虫C. andersoni，C. hominis和组合A-I，A-II，E of G.检测到尿道炎。必须监测牛、娱乐用水和农民使用的人工水道（水道、动物饮用水和排水系统）中这两种原生动物的存在。(C)2009爱思唯尔有限公司保留所有权利。
The objective of the present study was to determine the degree of contamination by Cryptosporidium spp. and Giardia duodenalis in a river basin in a livestock farming area in Galicia (NW, Spain). Water samples (50 l) were collected at 22 points in the main basin (including 5 recreational areas), and at the source and mouth of the 3 most important rivers and at the mouth of a smaller, secondary river. Faecal samples were collected from dairy cattle selected at random from 18 herds farmed in the area. A total of 139 neonatal calves, 480 heifers and 697 cows were sampled. The prevalence, intensity of infection and the risk associated with the spread of infection by both enteropathogens were determined. Water and faecal samples were collected in spring, summer, autumn and winter of 2007. The species and genotypes of these parasites present in the water samples were identified. in both water and faecal samples, more parasitic stages were collected in spring and summer than in autumn and winter. In spring, Cryptosporidium spp. oocysts were detected in 33 (9.4%) cows from 13 (72.2%) herds, and G. duodenalis cysts were detected in 56 (16.0%) cows from 15 farms (83.3%); the intensity of infection ranged from 5 to 7895 G. duodenalis cysts per gram of faeces. Infective stages of Cryptosporidium spp. and G. duodenalis were also detected in respectively 26 (89.6%) and 27 (93.1%) water samples, in spring. The mean concentrations of parasites ranged from 2 to 1200 Cryptosporidium spp. oocysts per litre and from 2 to 400 G. duodenalis cysts per litre. Cryptosporidium parvum, C. andersoni, C. hominis and assemblages A-I, A-II, E of G. duodenalis were detected. The presence of both protozoans must be monitored in cattle, in sources of water used for recreational purposes and in artificial waterways used by farmers (water channels, animal drinking water and drainage systems). (C) 2009 Elsevier Ltd. All rights reserved.