Influence of isomalto-oligosaccharides on intestinal microbiota in rats
Influence of isomalto-oligosaccharides on intestinal microbiota in rats
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DOI：
10.1111/j.1365-2672.2011.04984.x
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发表时间：
2011-05-01
影响因子：
4
通讯作者：
Gaenzle, M. G.
中科院分区：
文献类型：
--
作者：
Ketabi, A.;Dieleman, L. A.;Gaenzle, M. G.
目的：具有α(1 -> - 6)和α(1 -> - 4)糖苷键的异麦芽糖寡糖（IMO）是由淀粉酶转化产生的。IMO仅部分可消化，但其对肠道微生物群影响的数据有限。本研究旨在探讨IMO日粮对大鼠肠道菌群和短链脂肪酸生成的影响。方法与结果：F344大鼠3组，每组6只，分别饲喂IMO、菊粉或对照饲料6周。采用聚合酶链反应-变性梯度凝胶电泳（DGGE）对肠道菌群进行定性评价。采用定量PCR （qPCR）对主要细菌类群进行定量分析，采用气相色谱法对SCFA进行测定。定量PCR结果表明，乳酸菌是大鼠粪便中的优势菌群之一。与对照组和菊粉饲粮相比，饲喂IMO的大鼠乳酸菌数量和肠道细菌总数均有所增加。此外，使用乳酸菌特异性引物的PCR-DGGE显示，与对照组相比，饲喂IMO的大鼠的乳酸菌多样性发生了变化。结论：IMO可选择性刺激大鼠乳酸菌，增加其多样性。研究的意义和影响：异麦芽糖寡糖在大鼠模型系统中特异性刺激肠道乳酸菌的生长。
Aims:Isomalto-oligosaccharides (IMO) with alpha(1 -> 6) and alpha(1 -> 4) glucosidic linkages are produced by enzymatic conversion of starch. IMO are only partially digestible but data on their influence on intestinal microbiota are limited. It was the aim of this study to investigate the effect of IMO diet on intestinal microbiota and short-chain fatty acids production (SCFA) in rats.Methods and results:Three groups of F344 rats, each consisting of six animals, were fed IMO, inulin or a control diets for six weeks. A qualitative assessment of the intestinal microbiota was achieved by PCR-denaturing gradient gel electrophoresis (DGGE). Major bacterial taxa were quantified by quantitative PCR (qPCR), and SCFA were measured using gas chromatography. Quantitative PCR demonstrated that lactobacilli were one of the dominant bacterial taxa in faecal samples from rats. IMO increased the number of lactobacilli and the total number of intestinal bacteria in rats fed IMO compared with animals receiving control and inulin diets. Furthermore, PCR-DGGE with lactobacilli-specific primers showed an altered biodiversity of lactobacilli in rats fed IMO compared with control diet.Conclusions:IMO selectively stimulates lactobacilli and increases their diversity in rats.Significance and impact of study:Isomalto-oligosaccharides specifically stimulate growth of intestinal lactobacilli in a rat model system.