Organic Chemistry for Structural Biology : Probing the Functional Structure of Proteins by Photoaffinity Labeling
Organic Chemistry for Structural Biology : Probing the Functional Structure of Proteins by Photoaffinity Labeling
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结构生物学有机化学：通过光亲和标记探测蛋白质的功能结构
DOI：
10.5059/yukigoseikyokaishi.56.581
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发表时间：
1998
影响因子：
0.2
通讯作者：
Y. Hatanaka
中科院分区：
文献类型：
--
作者：
Y. Hatanaka
基于重组技术的最新发展，其配体接受界面的生物功能机制的研究已成为即将到来的新世纪有机化学的一个具有挑战性和重要的课题。目前用于结构生物学的三种主要方法都有各自的优点和局限性。光谱方法可用于分析原子水平上的配体-受体相互作用。然而，这些方法通常需要大量稳定且纯的蛋白质。基于基因技术的蛋白质工程为功能位点的结构分析提供了一系列突变体。使用这种方法的一个先决条件是突变体必须在很大程度上保留天然受体的构象。光亲和标记是第三种独立方法之一，作为一种用于配体结合域详细结构分析的强大化学方法越来越受到重视。光亲和标记技术是一种可靠的化学方法，应被视为与其他两种方法的补充而不是竞争。本综述重点关注光亲和标记在探测离子通道、受体和酶的功能结构方面的最新应用。
Based on the recent development of recombinant techniques, the investigation of biofunctional machinery at their ligand accepting interfaces has become a challenging and important subject of organic chemistry in the oncoming new century. Three major approaches currently used for structural biology have their own advantages and limitations. Spectroscopic methods are useful for analyzing ligand-receptor interactions at the atomic level. These approaches, however, usually require a significant amount of stable and pure proteins. Protein engineering based on gene technologies provides a series of mutants for the structural analysis of functional sites. One prerequisite for the use of this methods is that the mutants must, to a large extent, retain the conformation of the native receptors. Photoaffinity labeling, one of the third independent approach, has become increasingly appreciated as a powerful chemical methodology for the detailed structural analysis of ligand binding domains. The technique of photoaffinity labeling is a reliable chemical method which should be considered as being complementary to, rather than in competition with, the other two approaches. This review is focusing the recent application of photoaffinity labeling for probing the functional structure of ion channels, receptors, and enzymes.
2-叠氮基-ATP 对 Na,K-ATP 酶的光化学标记和抑制。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tran,CM;Huston,EE;Farley,RA
通讯作者：
Farley,RA