Effects of spinetoram on the developmental toxicity and immunotoxicity of zebrafish
Effects of spinetoram on the developmental toxicity and immunotoxicity of zebrafish
复制标题
多杀菌素对斑马鱼发育毒性和免疫毒性的影响
DOI：
10.1016/j.fsi.2019.11.066
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发表时间：
2020-01-01
影响因子：
4.7
通讯作者：
Lu, Huiqiang
中科院分区：
文献类型：
--
作者：
Cheng, Bo;Zhang, Hua;Lu, Huiqiang
本文研究了乙基多杀菌素对斑马鱼的发育毒性和免疫毒性。以受精后10 h的斑马鱼胚胎为研究对象，研究了不同浓度乙基多杀菌素（0、5.0 mg/L、7.5 mg/L、10 mg/L）对斑马鱼胚胎死亡率、心率、先天性和适应性免疫细胞数、氧化应激、细胞凋亡和基因表达的影响。研究表明，乙基多杀菌素处理后的斑马鱼胚胎出现卵黄囊水肿、生长缓慢、心率减慢、免疫细胞数量减少、胸腺发育延迟和细胞凋亡。此外，斑马鱼体内氧化应激相关指标也发生了显著变化，ROS和MDA含量以及CAT和SOD活性随着乙基多杀菌素浓度的增加而升高。另外，我们还检测了TLR 4相关基因的表达，包括TLR 4、MYD 88和NF-κ B B p65，这些基因在治疗组中表达显著上调。同时，我们还发现，在治疗组中，促炎因子IL-6、IL-8、IFN-γ和CXCL-c1 c表达上调，而抗炎因子IL-10表达下调。结果表明，乙基多杀菌素对斑马鱼具有一定的发育毒性和免疫毒性，为进一步研究乙基多杀菌素对水生生态系统的分子机制提供了依据。
Our study investigated the effects of spinetoram on the developmental toxicity and immunotoxicity of zebrafish. 10 h post-fertilization (hpf) zebrafish embryos were exposed to several concentrations of spinetoram (0, 5.0 mg/L, 7.5 mg/L, 10 mg/L) for up to 96 hpf, and their mortality, heart rate, number of innate and adaptive immune cells, oxidative stress, apoptosis and gene expression were detected. Studies indicated that the spinetoram exposed zebrafish embryos showed yolk sac edema, slow growth, decreased heart rate, decreased number of immune cells, delayed thymic development and cell apoptosis. In addition, there were also significant changes in oxidative stress related indicators in zebrafish, the content of ROS and MDA and the activity of CAT and SOD increased with the increase of spinetoram concentration. Moreover, we detected the expression of TLR4 related genes including TLR4, MYD88 and NF-kappa B p65 which were significantly up-regulated in the treated groups. Meanwhile, we also found that pro-inflammatory factors IL-6, IL-8, IFN-gamma and CXCL-c1c were up-regulated, but anti-inflammatory factor IL-10 was down-regulated in the treated groups. Briefly, our results show that spinetoram induces the developmental toxicity and immunotoxicity of zebrafish to a certain extent, providing basis for the further research on the molecular mechanism of spinetoram exposure to aquatic ecosystems.