Isolation of a Cryptococcus neoformans serotype A MATa strain from the Italian environment.
Isolation of a Cryptococcus neoformans serotype A MATa strain from the Italian environment.
复制标题
从意大利环境中分离出 A 型新型隐球菌 MATa 菌株。
DOI：
10.1080/mmy.39.5.383.386
复制
发表时间：
2001
期刊：
影响因子：
2.9
通讯作者：
Wickes,BL
中科院分区：
文献类型：
--
作者：
Viviani,MA;Esposto,MC;Cogliati,M;Montagna,MT;Wickes,BL
新型隐球菌（Cryptococcusneoformansis）是一种异宗配合的担子菌，具有基于两个交配型等位基因MAT α和MAT α的双极交配系统。在模式变种中，C.在血清型A的菌株中仅发现MAT α，而在血清型D的菌株中发现了MAT α。血清型AMATa似乎已经灭绝或仅以退化的、无功能的形式存在。我们报告的分离的aC。n.var.neoformansserotype AMATastrain来自意大利环境。采用玻片凝集试验进行血清学分型，采用（GACA）4引物进行聚合酶链反应（PCR）指纹图谱分析进行基因分型，并采用流式细胞术测定其单倍体状态。通过PCR扩增信息素鉴定交配型。此外，对血清型A和D交配型的4个STE 20等位基因的扩增表明，该菌株仅含有MATalocus。通过杂交实验发现该品系是可育的。发现该可育分离株的兴趣与构建用于遗传和致病性研究的血清型AMATa/MATα菌株的同源对的可能性有关。
Cryptococcus neoformansis a heterothallic basidiomycete which possesses a bipolar mating system based on two mating type alleles,MATaandMATα. In the type variety,C. neoformansvar.neoformans, both mating types have been found among strains of one serotype, serotype D, whereas onlyMATαwas identified after extensive survey of serotype A strains. Serotype AMATaappeared to be extinct or to exist only in a vestigial, non-functional form. We report the isolation of aC. n.var.neoformansserotype AMATastrain from the Italian environment. The strain was serotyped by slide agglutination test, genotyped by polymerase chain reaction (PCR) fingerprinting using the (GACA)4primer, and its haploid state was determined by flow cytometry. The mating type was identified by PCR amplification of the pheromoneagene. In addition, the amplification of the fourSTE20alleles, specific for the mating type of serotypes A and D, showed that the strain contains only theMATalocus. By crossing experiments the strain was found to be fertile. The interest in the finding of this fertile isolate is related to the possibility to construct a congenic pair of serotype AMATa/MATαstrains to be used in genetic and pathogenesis studies.