Profiling the endothelial function using both peripheral artery tonometry (EndoPAT) and Laser Doppler Flowmetry (LD) - Complementary studies or waste of time?
Profiling the endothelial function using both peripheral artery tonometry (EndoPAT) and Laser Doppler Flowmetry (LD) - Complementary studies or waste of time?
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DOI：
10.1016/j.mvr.2020.104008
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发表时间：
2020-07-01
影响因子：
3.1
通讯作者：
Doroszko, Adrian
中科院分区：
文献类型：
--
作者：
Jakubowski, Maciej;Turek-Jakubowska, Aleksandra;Doroszko, Adrian
内皮功能障碍在心血管疾病的发生发展中起着关键作用，是预测未来心血管事件的重要指标。然而，目前还没有确定的方法来评估普通人群的内皮功能。最流行的方案包括超声血流介导的扩张术，但其重复性取决于操作员。我们打算比较另外两种不依赖于操作员的评估内皮功能的方法--Endoat和激光多普勒血流仪(LD)，并努力将它们放在当前生化心血管风险和内皮功能的个体档案中。共调查61例临床健康受试者(年龄29+/-1岁)。检测常规心血管危险标志物、NO代谢产物(ADMA、L-精氨酸、SDMA)、氧化应激标志物(丙二醛、硫醇指数)以及内皮和血小板活化标志物(sICAM-1、sVCAM1、PAI-1、sE-选择素、sP-选择素、血管内皮生长因子)。随后，所有参与者都接受了Endoat和LD的检查。恩多巴和LD之间的相关性很差。内皮功能正常和受损的受试者在内皮激活和心血管危险标志物方面没有显著差异。这两种方法都独立于内皮细胞促炎/抗炎状态和常规危险因素来评估内皮功能，因此需要进一步的前瞻性研究，以验证它们在心血管危险分层中的附加价值。
Endothelial dysfunction (ED) plays a key role in developing of cardiovascular diseases and is an important predictor of future cardiovascular events. Nevertheless, there is no established method assessing endothelial function in general population. The most popular protocol includes the ultrasound-flow-mediated-dilation, but its repeatability is operator-dependent. We intended to compare the two other operator-independent methods assessing endothelial function - the EndoPAT and Laser Doppler flowmetry (LD), and we endeavored to place them on current individual profile of biochemical cardiovascular risk and endothelial function. A total of 61 clinically healthy subjects (aged 29 +/- 1y) were investigated. The blood was collected for conventional cardiovascular risk markers, the NO-pathway metabolites (ADMA, L-arginine, SDMA), oxidative-stress-markers (MDA, thiol-index) as well as endothelial and platelet activation markers (sICAM1, sVCAM1, PAI-1, sE-selectin, sP-selectin, VEGF). Subsequently, all participants underwent examination by both EndoPAT and LD. There was a poor correlation between EndoPAT and LD results. No significant differences between participants with preserved and impaired endothelial function regarding endothelial activation nor cardiovascular risk markers were observed. Both methods assess endothelial function independently from the profile of endothelial pro/anti-inflammatory status and conventional risk factors, therefore further prospective studies are needed in order to verify their additional value in the cardiovascular risk stratification.