Cutaneous Mechanical Stimulation Regulates Ovarian Blood Flow via Activation of Spinal and Supraspinal Reflex Pathways in Anesthetized Rats.
Cutaneous Mechanical Stimulation Regulates Ovarian Blood Flow via Activation of Spinal and Supraspinal Reflex Pathways in Anesthetized Rats.
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DOI：
10.2170/jjphysiol.r2133
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发表时间：
2005-10
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影响因子：
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通讯作者：
S. Uchida;F. Kagitani;H. Hotta;Tomoko Hanada;Yoshihiro Aikawa
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文献类型：
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作者：
S. Uchida;F. Kagitani;H. Hotta;Tomoko Hanada;Yoshihiro Aikawa
在麻醉大鼠中检查了后爪或腹部皮肤的伤害性机械刺激对卵巢血流的反射效应，以及参与这些反应的反射途径。采用激光多普勒血流仪测量左侧卵巢血流，记录左侧卵巢交感神经活性和颈总动脉平均动脉压（MAP）。刺激左或右后爪30 s，卵巢交感神经活动和MAP显著增加。卵巢血流量在刺激期间略有下降，然后在刺激后略有增加。在左侧卵巢交感神经被切断后，同样的刺激产生了一个显着的卵巢血流量的单向增加，这是由于MAP显着增加的被动血管舒张解释。在第三胸（T3）水平脊髓横断后，MAP，卵巢交感神经活动和卵巢血流对后爪刺激的反应几乎消失。刺激右侧或左侧腹部30 s，卵巢交感神经活动和MAP略有增加。卵巢血流量在刺激期间略有下降，然后在刺激后略有增加。卵巢交感神经切断后，由于MAP升高，卵巢血流反应变为单相增加。脊髓横断后，刺激左腹部产生了适度的MAP增加，卵巢交感神经活动显着增加，卵巢血流量略有减少，在刺激过程中。与此相反，刺激右腹部产生较小的反应，在卵巢交感神经活动的刺激，而它增加了MAP到类似的程度。刺激结束后卵巢血流量略有增加，这被解释为由于MAP增加而导致的被动血管舒张。总之，躯体传入刺激通过引起卵巢交感神经活动和血压的变化来影响卵巢血流。当刺激后爪的传入神经段远离卵巢交感神经段时，它走脊髓上反射通路。而当刺激位于脊髓段传入神经与卵巢交感神经输出神经段距离较近的腹部时，则存在脊髓段反射通路。本研究结果表明，脊髓反射依赖于刺激的偏侧性，而脊髓上反射不依赖于刺激的偏侧性。
The reflex effects of noxious mechanical stimulation of a hindpaw or abdominal skin on ovarian blood flow, and the reflex pathways involved in those responses were examined in anesthetized rats. Blood flow in the left ovary was measured using a laser Doppler flowmeter, and the activity of the left ovarian sympathetic nerve and mean arterial pressure (MAP) of the common carotid artery were recorded. Stimulation of the left or right hindpaw for 30 s produced marked increases in ovarian sympathetic nerve activity and MAP. Ovarian blood flow slightly decreased during the stimulation and then slightly increased after the stimulation. After the left ovarian sympathetic nerves were severed, the same stimulus produced a remarkable monophasic increase in ovarian blood flow that was explained by passive vasodilation due to a marked increase in MAP. After spinal transection at the third thoracic (T3) level, the responses of MAP, ovarian sympathetic nerve activity, and ovarian blood flow to hindpaw stimulation were nearly abolished. Stimulation of the abdomen at the right or left side for 30 s produced slight increases in ovarian sympathetic nerve activity and MAP. Ovarian blood flow slightly decreased during the stimulation and then slightly increased after the stimulation. After the ovarian sympathetic nerves were severed, the response of the ovarian blood flow changed to a monophasic increase due to an increase in MAP. After spinal transection, stimulation of the left abdomen produced a moderate increase in MAP, a remarkable increase in ovarian sympathetic nerve activity and a slight decrease in ovarian blood flow during the stimulation. In contrast, stimulation of the right abdomen produced a smaller response in ovarian sympathetic nerve activity during the stimulation while it increased the MAP to a similar degree. Ovarian blood flow slightly increased after the end of stimulation, which was explained as passive vasodilation due to the increase in MAP. In conclusion, stimulation of somatic afferents affects ovarian blood flow by inducing changes in ovarian sympathetic nerve activities and blood pressure. When stimulation was applied to a hindpaw whose segment of afferent input is far from the segment of the ovarian sympathetic nerves, it took a supraspinal reflex pathway. However, when stimulation was applied to the abdomen whose spinal segment of the afferent is close to the segment of the ovarian sympathetic nerve output, there are spinal segmental reflex pathways. The present results demonstrate that spinal reflexes depend on the laterality of the stimulus, while supraspinal reflexes do not depend on the laterality of the stimulus.