STRUCTURE AND ORGANIZATION OF THE HEPATITIS-C VIRUS GENOME ISOLATED FROM HUMAN CARRIERS
STRUCTURE AND ORGANIZATION OF THE HEPATITIS-C VIRUS GENOME ISOLATED FROM HUMAN CARRIERS
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DOI：
10.1128/jvi.65.3.1105-1113.1991
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发表时间：
1991-03-01
影响因子：
5.4
通讯作者：
OKAYAMA, H
中科院分区：
文献类型：
--
作者：
TAKAMIZAWA, A;MORI, C;OKAYAMA, H
丙型肝炎病毒（HCV）是输血后非甲、非乙型肝炎的主要病原体，常发展为恶性慢性疾病，包括肝硬化和肝细胞癌。我们从人类携带者中克隆了覆盖整个HCV基因组编码区的重叠cdna （9416 bp）。后者编码3010个氨基酸的多蛋白。此外，5′和3′非编码序列分别有332个碱基和54个碱基。我们的HCV病毒株与Chiron公司工人克隆的HCV病毒株具有77%的核酸同源性。推测的多蛋白的疏水性与黄病毒相似，但与黄病毒和其他病毒的多蛋白氨基酸同源性有限，表明HCV与黄病毒的亲缘关系最远。
Hepatitis C virus (HCV) is a major causative agent of posttransfusion non-A, non-B hepatitis, which often develops into malignant chronic diseases, including liver cirrhosis and hepatocellular carcinoma. We have cloned from human carriers overlapping cDNAs (9,416 bp) covering the entire coding region of the HCV genome. The latter encodes a 3,010-amino-acid polyprotein. In addition, there are 332 and 54 bases of 5' and 3' noncoding sequences, respectively. Our HCV strain has a 77% nucleic acid identity to the HCV strain cloned by workers at Chiron Corporation. The hydrophobicity profile of the putative polyprotein is similar to those of flaviviruses, but it has limited amino acid homology to polyproteins of flaviviruses and other viruses, indicating that HCV is at most distantly related to flaviviruses.