Macro-micro imaging of cardiac-neural circuits in co-cultures from normal and diseased hearts.
Macro-micro imaging of cardiac-neural circuits in co-cultures from normal and diseased hearts.
复制标题
正常和患病心脏共培养物中心脏神经回路的宏观显微成像。
DOI：
10.1113/jphysiol.2014.285460
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Bub G
中科院分区：
文献类型：
--
作者：
Bub G
摘要自主神经系统在调节正常心律中起着重要作用，但也参与调节心脏对折返性室性和房性心律失常的易感性。自主神经系统促心律失常或抗心律失常的机制是多方面的，不同类型的心律失常及其心脏底物是不同的。尽管在这一领域进行了数十年的研究，但有关神经元密度和空间组织如何调节心电波动力学的基本问题仍未得到解答。这些问题在完整的组织中可能是不合适的，因为在这些组织中，单个细胞的活动通常在实验上是无法获得的。开发简化的生物学模型，使我们能够更好地了解神经激活对心脏活动的影响可能是有益的。本研讨会综述了折返活性的体外心肌细胞培养模型的研究进展，以及将这些模型扩展到包括神经激活的影响所面临的挑战。
AbstractThe autonomic nervous system plays an important role in the modulation of normal cardiac rhythm, but is also implicated in modulating the heart's susceptibility to re‐entrant ventricular and atrial arrhythmias. The mechanisms by which the autonomic nervous system is pro‐arrhythmic or anti‐arrhythmic is multifaceted and varies for different types of arrhythmia and their cardiac substrates. Despite decades of research in this area, fundamental questions related to how neuron density and spatial organization modulate cardiac wave dynamics remain unanswered. These questions may be ill‐posed in intact tissues where the activity of individual cells is often experimentally inaccessible. Development of simplified biological models that would allow us to better understand the influence of neural activation on cardiac activity can be beneficial. This Symposium Review summarizes the development ofin vitrocardiomyocyte cell culture models of re‐entrant activity, as well as challenges associated with extending these models to include the effects of neural activation.