Evidence for interleukin-10-mediated inhibition of cyclo-oxygenase-2 expression and prostaglandin production in preterm human placenta
Evidence for interleukin-10-mediated inhibition of cyclo-oxygenase-2 expression and prostaglandin production in preterm human placenta
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DOI：
10.1111/j.1600-0897.2005.00342.x
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发表时间：
2006-01-01
影响因子：
3.6
通讯作者：
Sharma, S
中科院分区：
文献类型：
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作者：
Hanna, N;Bonifacio, L;Sharma, S
白细胞介素-10（IL-10）被认为是维持妊娠的关键细胞因子。在这里，我们研究了IL- 10和环氧合酶-2（考克斯-2）的表达谱，以及IL-10对与绒毛膜炎相关的早产分娩的人胎盘中考克斯-2表达和前列腺素释放的影响。处理来自早产和足月分娩的胎盘组织用于离体胎盘外植体培养系统。通过酶联免疫吸附试验（ELISA）和免疫组织化学（IHC）分析评估IL-10表达。免疫组化、Western blotting和逆转录聚合酶链反应检测考克斯-2的表达。通过ELISA法测定前列腺素E-2（PGE（2））的释放。结果。IL-10显着降低绒毛膜炎相关的早产，以及在足月分娩胎盘组织相比，孕中期正常妊娠样本从选择性终止。从这些分娩中新鲜分离的细胞滋养层细胞获得了类似的结果。正如所料，考克斯-2 mRNA在足月分娩和早产分娩的组织中检测到显著水平，与无足月分娩相比。重要的是，IL-10抑制早产分娩胎盘组织培养块中考克斯-2的表达，但不抑制足月分娩样本中的表达。考克斯-2表达的抑制与PGE（2）释放的减少一致。这些结果表明IL-10在对抗与早产相关的炎症中的重要性，并表明足月分娩和早产可能部分代表不同的条件。
Problem Interleukin-10 (IL-10) is thought to be a key cytokine for the maintenance of pregnancy. Here we examined the expression profiles of IL- 10 and cyclo-oxygenase-2 (COX-2), and the effect of IL-10 on COX-2 expression and prostaglandin release in the human placenta from pre-term labor deliveries associated with chorioamnionitis.Method of study. Placental tissues from preterm labor and term labor deliveries were processed for ex vivo placental explant culture system. IL-10 expression was assessed by enzyme-linked immunosorbent assay (ELISA) and immunohistochemical (IHC) analysis. COX-2 expression was evaluated by IHC, Western blotting and reverse transcriptase- polymerase chain reaction. Prostaglandin E-2 (PGE(2)) release was measured by ELISA.Results. IL-10 was significantly reduced in chorioamnionitis-associated preterm labor as well as in term labor placental tissues compared with second trimester normal pregnancy samples obtained from elective terminations. Similar results were obtained with freshly isolated cytotrophoblasts from these deliveries. As expected, COX-2 mRNA was detected at significant levels in tissues from term and preterm labor deliveries compared with no labor term deliveries. Importantly, IL-10 inhibited COX-2 expression in cultured placental explants from preterm labor deliveries, but not from term labor samples. Inhibition of COX-2 expression coincided with reduced PGE(2) release.Conclusion. These results demonstrate the importance of IL-10 in countering inflammation associated with preterm labor, and suggest that term and preterm parturition may, in part, represent different conditions.