Expression of circadian clock gene human Period2 (hPer2) in human colorectal carcinoma.
Expression of circadian clock gene human Period2 (hPer2) in human colorectal carcinoma.
复制标题
生物钟基因人Period2 (hPer2)在人结直肠癌中的表达
DOI：
10.1186/1477-7819-9-166
复制
发表时间：
2011-12-13
影响因子：
3.2
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Wang Y;Hua L;Lu C;Chen Z
背景
最近的研究表明，昼夜节律的紊乱是哺乳动物肿瘤发生发展的促癌因素之一，但对结直肠癌中昼夜节律基因的分子变化知之甚少。因此，本研究旨在探讨人周期蛋白2(HPer2)在结直肠癌中的表达变化及其与预后的关系。
方法
对38例大肠癌组织进行免疫组织化学(IHC)染色和实时定量聚合酶链式反应(RT-PCR)检测hPer2。
结果
免疫组化检测hPer2在81.6%(31/38)的大肠癌组织和97.4%(37/38)的癌旁组织中呈阳性表达(P<0.05)。非癌组织中的大部分结直肠癌细胞染色均匀。相反，在配对的癌组织中，发现了一种不同的模式，相当一部分癌细胞显示hPer2染色阴性或微弱。在超过60%(24/38)的病例中，hPer2在非癌细胞中的表达明显强于配对的癌细胞。高分化的癌细胞比低分化的癌细胞更有可能保持hPer2的表达。此外，hPer2水平的降低还与患者的年龄、组织学分级、TNM分期和核增殖相关抗原Ki67的表达有关(P<0.05)。HPer2的表达与P53、C-Erb-2的表达均无相关性。与hPer2蛋白表达类似，定量RT-PCR也显示hPer2在结直肠癌中的mRNA表达降低。
结论
这些结果提示hPer2在正常的结直肠细胞功能中的作用，以及hPer2表达在结直肠癌的发生、侵袭和转移中的潜在失控。
Background
Recent studies have shown that disruption of circadian rhythms is one of the tumor promoting factors which contribute to mammalian cancer development and progression, but very little is known about the molecular changes of circadian genes in colorectal carcinoma (CRC). Thus, in this study, changes in the expression of human Period2 (hPer2), one of the key circadian clock regulators, in CRC and its correlation with prognosis were investigated.
Methods
Immunohistochemical (IHC) staining and real-time PCR for hPer2 were performed for 38 CRC cases.
Results
IHC analysis detected positive staining for hPer2 in 81.6% (31/38) of CRC tissues and 97.4% (37/38) of surrounding non-cancerous tissues (P 0.05). Most colorectal cells in non-cancerous tissues were homogeneously stained. In contrast, in the paired cancerous tissues, a heterogeneous pattern was found with a significant portion of cancer cells displaying negative or weak hPer2 staining. In over 60% cases (24/38), the staining for hPer2 was much stronger in non-cancerous cells than in the paired cancerous cells. Well-differentiated cancer cells are more likely to maintain hPer2 expression than poorly-differentiated ones. Furthermore, associations of decreased hPer2 levels with patients' age, histological grade, TNM stage and expression of nucleus proliferation related antigen: Ki67 were also detected (P 0.05). Expression of hPer2 did not correlate with that of either p53 or C-erB-2. Similar to hPer2 protein expression, quantitative RT-PCR for hPer2 also showed decreased mRNA expression in CRC.
Conclusion
These results suggest a role for hPer2 in normal colorectal cell function and the potential deregulation of hPer2 expression in the development, invasion, and metastasis of CRC.
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影响因子：
4.7
作者：
Chen, ST;Choo, KB;Chang, JG
通讯作者：
Chang, JG
影响因子：
2.8
作者：
Giacchetti, S
通讯作者：
Giacchetti, S
DOI：
10.1093/jnci/92.12.987
发表时间：
2000-06-21
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
Orr, MS;O'Connor, PM;Kohn, KW
通讯作者：
Kohn, KW
影响因子：
2.3
作者：
Lee, CC
通讯作者：
Lee, CC
影响因子：
29.4
作者：
Karnes, WE;Weller, SG;Kaufmann, SH
通讯作者：
Kaufmann, SH