Effects of low-energy argon ion implantation on the dynamic organization of the actin cytoskeleton during maize pollen germination.
Effects of low-energy argon ion implantation on the dynamic organization of the actin cytoskeleton during maize pollen germination.
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DOI：
10.4238/vol9-2gmr768
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发表时间：
2010-04
期刊：
影响因子：
--
通讯作者：
F. Deng;S. Zhu;L. Wu;B. Cheng
中科院分区：
文献类型：
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作者：
F. Deng;S. Zhu;L. Wu;B. Cheng
花粉萌发与花粉萌发过程中肌动蛋白细胞骨架的动态变化之间的关系是植物生殖生物学研究的一个中心主题。用30 keV氩离子（Ar（+））束以0.78 × 10 ~（15）~ 13 × 10 ~（15）ions/cm ~ 2的剂量注入玉米花粉粒。利用激光共聚焦扫描显微镜研究了低能离子注入对花粉萌发活力和萌发过程中肌动蛋白细胞骨架动态组织的影响。在3.9 × 10 ~（15）~ 6.5 × 10 ~（15）ions/cm ~ 2范围内，玉米花粉萌发率随Ar（+）剂量的增加而显著增加，在Ar（+）剂量为5.2 × 10 ~（15）ions/cm ~ 2时萌发率达到最高。当注入剂量超过7.8 × 10 ~（15）ions/cm ~ 2时，花粉萌发率急剧下降。注入5.2 × 10 ~（15）ions/cm ~（2）Ar（+）的花粉粒中肌动蛋白丝的组装比对照早得多。处理花粉的肌动蛋白丝排列成平行的长束，并延伸到萌发的花粉管中，而对照花粉的肌动蛋白丝排列成松散的细束，并聚集在花粉孔中。注入9.1 × 10（15）ions/cm（2）Ar（+）的花粉中肌动蛋白细胞骨架的重组比对照慢。花粉萌发与花粉萌发过程中肌动蛋白细胞骨架的动态变化呈正相关。离子注入花粉未引起花粉管中肌动蛋白丝极化和细胞器动态的变化。Ar（+）离子注入对花粉萌发的影响可能是通过改变肌动蛋白聚合体的聚合和重排来介导的。
The relationship between pollen germination and the dynamic organization of the actin cytoskeleton during pollen germination is a central theme in plant reproductive biology research. Maize (Zea mays) pollen grains were implanted with 30 keV argon ion (Ar(+)) beams at doses ranging from 0.78 x 10(15) to 13 x 10(15) ions/cm(2). The effects of low-energy ion implantation on pollen germination viability and the dynamic organization of the actin cytoskeleton during pollen germination were studied using confocal laser scanning microscopy. Maize pollen germination rate increased remarkably with Ar(+) dose, in the range from 3.9 x 10(15) to 6.5 x 10(15) ions/cm(2); the germination rate peaked at an Ar(+) dose of 5.2 x 10(15) ions/cm(2). When the implantation dose exceeded 7.8 x 10(15) ions/cm(2), the rate of pollen germination decreased sharply. The actin filaments assembled in pollen grains implanted with 5.2 x 10(15) ions/cm(2) Ar(+) much earlier than in controls. The actin filaments organized as longer parallel bundles and extended into the emerging pollen tube in treated pollen grains, while they formed random and loose fine bundles and were gathered at the pollen aperture in the control. The reorganization of actin cytoskeleton in the pollen implanted with 9.1 x 10(15) ions/cm(2) Ar(+) was slower than in controls. There was a positive correlation between pollen germination and the dynamic organization of the actin cytoskeleton during pollen germination. Ion implantation into pollen did not cause changes in the polarization of actin filaments and organelle dynamics in the pollen tubes. The effects of Ar(+) implantation on pollen germination could be mediated by changes in the polymerization and rearrangement of actin polymers.