COMMON MECHANISM CONTROLLING PHASE AND ANTIGENIC VARIATION IN PATHOGENIC NEISSERIAE
COMMON MECHANISM CONTROLLING PHASE AND ANTIGENIC VARIATION IN PATHOGENIC NEISSERIAE
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DOI：
10.1111/j.1365-2958.1987.tb00520.x
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发表时间：
1987-07-01
影响因子：
3.6
通讯作者：
MEYER, TF
中科院分区：
文献类型：
--
作者：
STERN, A;MEYER, TF
淋病奈瑟菌不透明蛋白(Op，Protein II)是淋病病毒外膜的主要抗原决定簇，其表达经历了频繁的相变。至少有9个表达位点(OpE)参与了大量血清学上不同的Op类型的产生。在基因组印迹中使用OPA特异的寡核苷酸作为探针，我们检测到Op相关基因序列(Opr)。脑膜炎诉讼以及客栈。内酰胺酶基因的DNA序列分析。脑膜炎揭示了与淋球菌基因有明显同源性的区域。免疫印迹显示，由ops和opr编码的蛋白质在血清学上是相关的。与OPA E基因一样，Theoprgene的5‘-编码序列包括由五聚体CTT单位组成的重复序列。这些编码重复(CR)单元的数量是可变的。这一发现，再加上观察到的异源基因是结构性转录的，无论蛋白质产生的状态如何，都表明了一种翻译控制机制，与淋球菌中的Theopagenes相同。这些基因的相关结构和调控机制表明，它们的基因产物对于所研究的奈瑟氏菌的致病特性具有普遍的意义。
The expression of theNeisseria gonorrhoeaeopacity protein (Op, protein II), a major antigenic determinant of the outer membrane, is subject to frequent phase transitions. At least nine expression loci (opaE) are involved in the production of a large number of serologically distinct Op types. Using opa‐specific oligonucleotides as probes in genomic blots, we detect Op‐related gene sequences (opr) inN. meninglitidisas well as inN. lactamica.DNA sequence analysis of suchoprgenes derived fromN. meninglitidisreveals distinct regions of homology with gonococcalopaE genes. As shown in the immunoblot, the proteins encoded by ops and opr are serologically related. Like the opa E genes, the 5′‐coding sequences of theoprgenes include a repetitive sequence composed of pentameric CTCTT units. The number of these coding repeat (CR) units is variable. This finding, together with the observation that alloprgenes are constitutively transcribed, regardless of the status of protein production, suggests a translational control mechanism identical to that of theopagenes in gonococci. The related structures and control mechanisms ofopaandoprgenes imply a general significance of their gene products for the pathogenic character of the investigatedNeisseriaspecies.