Colistin-Resistant Acinetobacter baumannii Clinical Strains with Deficient Biofilm Formation
Colistin-Resistant Acinetobacter baumannii Clinical Strains with Deficient Biofilm Formation
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DOI：
10.1128/aac.02518-15
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发表时间：
2016-03-01
影响因子：
4.9
通讯作者：
Pournaras, Spyros
中科院分区：
文献类型：
--
作者：
Dafopoulou, Konstantina;Xavier, Basil Britto;Pournaras, Spyros
在两对鲍曼不动杆菌临床粘菌素敏感/耐药菌株(Cst（s)/Cst(r)）中，与Cst(s)相比，Cst(r)菌株在静态和动态试验中生物膜形成显著减少（P < 0.001），相对适应度较低（P < 0.05）。菌株对的全基因组测序比较发现，在一个Cst(r)菌株中，LpsB（参与脂多糖[LPS]合成）中的终止密码子转化为赖氨酸（*241K）发生突变，在CarO中发生移码突变，另一个Cst(r)菌株中丢失了一个47,969 bp的元件，该元件包含与生物膜生成相关的多个基因。
In two pairs of clinical colistin-susceptible/colistin-resistant (Cst(s)/Cst(r)) Acinetobacter baumannii strains, the Cst(r) strains showed significantly decreased biofilm formation in static and dynamic assays (P < 0.001) and lower relative fitness (P < 0.05) compared with those of the Cst(s) counterparts. The whole-genome sequencing comparison of strain pairs identified a mutation converting a stop codon to lysine (*241K) in LpsB (involved in lipopolysaccharide [LPS] synthesis) in one Cst(r) strain and a frameshift mutation in CarO and the loss of a 47,969-bp element containing multiple genes associated with biofilm production in the other.