ATTACHING AND EFFACING ACTIVITIES OF RABBIT AND HUMAN ENTEROPATHOGENIC ESCHERICHIA-COLI IN PIG AND RABBIT INTESTINES
ATTACHING AND EFFACING ACTIVITIES OF RABBIT AND HUMAN ENTEROPATHOGENIC ESCHERICHIA-COLI IN PIG AND RABBIT INTESTINES
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DOI：
10.1128/iai.41.3.1340-1351.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
GIANNELLA, RA
中科院分区：
文献类型：
--
作者：
MOON, HW;WHIPP, SC;GIANNELLA, RA
三株肠致病性大肠杆菌（EPEC），最初从人类中分离出来，以前显示以未知的机制引起人类志愿者腹泻，1株家兔EPEC菌株密切附着在猪和兔肠道上皮细胞的微绒毛和细胞质上。通过常规组织学切片的光镜检查和透射电镜检查可以证明这些EPEC的附着和去除活性。完整的初乳被剥夺的新生猪和猪、兔结扎的肠环可能是检测具有附着和清除活性的EPEC和研究此类感染的发病机制的有用系统。EPEC在这些系统中产生的病变（附着和消失）是多灶性的，在同一株EPEC的反应中，动物与动物之间存在相当大的差异。EPEC菌株在病变产生的频率和程度上也各不相同。三种人EPEC菌株通常在家兔肠袢中引起广泛病变；另外2种人EPEC菌株通常在该系统中不产生病变。
Three strains of enteropathogenic E. coli (EPEC), originally isolated from humans and previously shown to cause diarrhea in human volunteers by unknown mechanisms, and 1 rabbit EPEC strain attached intimately to an effaced microvilli and cytoplasm from intestinal epithelial cells in the pig and rabbit intestine. The attaching and effacing activities of these EPEC were demonstrable by light microscopic examination of routine histological sections and by transmission electron microscopy. Intact colostrum-deprived newborn pigs and ligated intestinal loops in pigs and rabbits may be useful systems to detect EPEC that have attaching and effacing activities and for studying the pathogenesis of such infections. The lesions (attachment and effacement) produced by EPEC in these systems were multifocal, with considerable animal-to-animal variation in response to the same strain of EPEC. The EPEC strains also varied in the frequency and extent of lesion production. Three human EPEC strains usually caused extensive lesions in rabbit intestinal loops; 2 other human EPEC strains usually did not produce lesions in this system.