Cell differentiation guidance using chemical stimulation controlled by a microfluidic device
Cell differentiation guidance using chemical stimulation controlled by a microfluidic device
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DOI：
10.1016/j.sna.2007.05.035
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发表时间：
2007-09-12
影响因子：
4.6
通讯作者：
Yasuda, Takashi
中科院分区：
文献类型：
--
作者：
Nakashima, Yuta;Yasuda, Takashi
本文介绍了一种利用微流控装置控制细胞分化的试验。该微流控装置由细胞培养腔、微通道、微阀和纳米孔阵列组成。由于这些部件的制造非常接近，所以微型阀开关可以精确控制化学物质的释放。因此，培养在设备上的细胞通过纳米孔阵列直接受到化学物质的刺激。我们在研制的微流控装置上培养了PC12细胞(肾上腺嗜铬细胞瘤)，并利用神经生长因子(NGF)进行了细胞分化诱导实验，神经生长因子促进了细胞体的轴突生长。这些结果表明，细胞的生长依赖于细胞与纳米孔阵列之间的距离。此外，瓣膜切换的频率和占空比对细胞分化和轴突伸长也有重要影响。因此，我们可以通过使用所制造的装置来控制它们来指导细胞分化和轴突延长。(C)2007 Elsevier B.V.保留所有权利。
This paper presents the testing of a cell differentiation control using a microfluidic device. The microfluidic device consists of a cell culture chamber, a microchannel, a microvalve, and a nano-hole array. Because these components are fabricated very close to one another, the microvalve switching allows precise control for releasing chemicals. Therefore, a cell cultured on the device is stimulated directly by chemicals through the nano-hole array. We cultured PC12 cells (adrenal pheochromocytoma) on the fabricated microfluidic device, and carried out experiments of cell differentiation guidance using the nerve growth factor (NGF) which enhances axonal outgrowth from a cell body. These results indicate that the cell growth depended on distance between the cell and the nano-hole array. Also, the cell differentiation and the axon elongation were affected materially by the frequency and the duty factor of the valve switching. Therefore, we can guide cell differentiation and axon elongation by controlling them using the fabricated device. (c) 2007 Elsevier B.V. All rights reserved.